Bi-Chang Chen


  • 1997~2001 B.S., Department of Chemistry, National Taiwan University, Taipei, Taiwan,
  • 2001~2003 M.S., Department of Chemistry, National Taiwan University, Taipei, Taiwan
  • 2006~2011 Ph. D., Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX, USA

Research Fields

  • Light Sheet Microscopy
  • Developing fast, low phototoxicity, multi-color, and 3D detection for fluorescent living specimens with subcellular resolution imaging tools
  • Applications ranging from single molecule tracking to high spatiotemporal imaging of subcellular dynamics within whole developing embryos.


  • 2020 April ~ present Associate Research Fellow, Research Center for Applied Sciences, Academia Sinica, Taiwan
  • 2014 ~ 2020 April Assistant Research Fellow, Research Center for Applied Sciences, Academia Sinica, Taiwan
  • 2011~ 2014 Mar Postdoctoral Associate, Howard Hughes Medical Institute/Janelia Farm Research Campus, USA



Development of a water refractive index-matched microneedle integrated into a light sheet microscopy system for continuous embryonic cell imaging
Fan, Y.-J.*, Hsieh, H.-Y., Huang, Y.-R., Tsao, C., Lee, C.-M., Tahara,H., Wu,Y.-C., Sheen, H.-J.*, (Chen, B.-C.*)
 [ DOI here ]    Year:2022-01-17
Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain
Lin Ya-Hui, Wang Li-Wen, Chen Yen-Hui, Chan Yi-Chieh, Hu Shang-Hsiu, Wu Sheng-Yan, Chiang Chi-Shiun, Huang Guan-Jie, Yang Shang-Da, Chu Shi-Wei, Wang Kuo-Chuan, Lin Chin-Hsien, Huang Pei-Hsin, Cheng Hwai-Jong, (Chen Bi-Chang)*, Chu Li-An*
 [ DOI here ]    Year:2024-01-15
Protein and lipid expansion microscopy with trypsin and tyramide signal amplification for 3D imaging
Wang Ueh-Ting Tim, Tian Xuejiao, Liou Yae-Huei, Lee Sue-Ping, Hu Hsiao-Tang, Lu Chieh-Han, Lin Po-Ting, Cheng Ya-Jen, Chen Peilin, Chen Bi-Chang
 [ DOI here ]    Year:2023-12-08
Scientific Reports
Autism-related KLHL17 and SYNPO act in concert to control activity-dependent dendritic spine enlargement and the spine apparatus
Hu Hsiao-Tang, Lin Yung-Jui, Wang Ueh-Ting Tim, Lee Sue-Ping, Liou Yae-Huei, Chen Bi-Chang, Hsueh Yi-Ping
 [ DOI here ]    Year:2023-08-31
PLOS Biology
SARS-CoV-2 N protein mediates intercellular nucleic acid dispersion, a feature reduced in Omicron
Wu Jung-Lin, Kuan I.-I., Guo Jing-You, Hsu Wei-Chia, Tang Wei-Chun, Chan Hsin-Ju, Chen Yu-Ju, Chen Bi-Chang, Wu Han-Chung, Liao James C.
 [ DOI here ]    Year:2023-02-17
Large-scale expanded sample imaging with tiling lattice lightsheet microscopy
Lu Chieh-Han, Huang Cheng-Yu, Tian Xuejiao, Chen Peilin, (Chen Bi-Chang*)
 [ DOI here ]    Year:2023-01-15
The International Journal of Biochemistry & Cell Biology
Optogenetic manipulation of cell migration with high spatiotemporal resolution using lattice lightsheet microscopy
Tang Wei-Chun, Liu Yen-Ting, Yeh Cheng-Han, Lu Chieh-Han, Tu Chiao-Hui, Lin Yi-Ling, Lin Yu-Chun, Hsu Tsui-Ling, Gao Liang, Chang Shu-Wei, Chen Peilin*, (Chen Bi-Chang*)
 [ DOI here ]    Year:2022-8-26
Communications Biology
Neuronal paxillin and drebrin mediate BDNF-induced force transduction and growth cone turning in a soft-tissue-like environment
Chen Chen, Chu Chien-Hsin, Chu Ying, Chang Ting-Ya, Chen Sheng-Wen, Liang Shu-Yang, Tsai Yun-Chi, (Chen Bi-Chang), Tu Hsiung-Lin, Cheng Pei-Lin
 [ DOI here ]    Year:2022-8
Cell Reports
High-resolution spatial and genomic characterization of coral-associated microbial aggregates in the coral Stylophora pistillata
Wada Naohisa, Hsu Ming-Tsung, Tandon Kshitij, Hsiao Silver Sung-Yun, Chen Hsing-Ju, Chen Yu-Hsiang, Chiang Pei-Wen, Yu Sheng-Ping, Lu Chih-Ying, Chiou Yu-Jing, Tu Yung-Chi, Tian Xuejiao, (Chen Bi-Chang), Lee Der-Chuen, Yamashiro Hideyuki, Bourne David G., Tang Sen-Lin
 [ DOI here ]    Year:2022-7-8
Science Advances
Macrophages Break Interneuromast Cell Quiescence by Intervening in the Inhibition of Schwann Cells in the Zebrafish Lateral Line
Lin Meng-Ju, Lee Chia-Ming, Hsu Wei-Lin, (Chen Bi-Chang), Lee Shyh-Jye
 [ DOI here ]    Year:2022-7-1
Frontiers in Cell and Developmental Biology
GSK3α phosphorylates dynamin-2 to promote GLUT4 endocytosis in muscle cells
Laiman Jessica, Hsu Yen-Jung, Loh Julie, Tang Wei-Chun, Chuang Mei-Chun, Liu Hui-Kang, Yang Wei-Shun, Chen Bi-Chang, Chuang Lee-Ming, Chang Yi-Cheng, Liu Ya-Wen
 [ DOI here ]    Year:2022-11-29
Journal of Cell Biology
5D superresolution imaging for a live cell nucleus
Chu Li-An, Chang Shu-Wei, Tang Wei-Chun, Tseng Yu-Ting, Chen Peilin, (Chen Bi-Chang*)
 [ DOI here ]    Year:2021
Current Opinion in Genetics & Development
Impairment in renal medulla development underlies salt wasting in Clc-k2 channel deficiency
Lin Meng-Hsuan, Chen Jen-Chi, Tian Xuejiao, Lee Chia-Ming, Yu I-Shing, Lo Yi-Fen, Uchida Shinichi, Huang Chou-Long, (Chen Bi-Chang*), Cheng Chih-Jen
 [ DOI here ]    Year:2021
JCI Insight
Macro Photography with Lightsheet Illumination Enables Whole Expanded Brain Imaging with Single-cell Resolution
Chia-Ming Lee, Xuejiao Tian, Chieh Tsao, Peilin Chen, Tzyy-Nan Huang, Yi-Ping Hsueh, (Bi-Chang Chen*)
 [ DOI here ]    Year:2021
Discoveries Journal
Microfluidic channel integrated with a lattice lightsheet microscopic system for continuous cell imaging
Fan Yu-Jui*, Hsieh Han-Yun, Tsai Sheng-Fang, Wu Cheng-Hsuan, Lee Chia-Ming, Liu Yen-Ting, Lu Chieh-Han, Chang Shu-Wei*, (Chen Bi-Chang*)
 [ DOI here ]    Year:2021
Lab on a Chip
Two-photon scanned light sheet fluorescence microscopy with axicon imaging for fast volumetric imaging
Lin Po-Yen, Hwang Sheng-Ping L., Lee Chi-Hon*, (Chen Bi-Chang*)
 [ DOI here ]    Year:2021
Journal of Biomedical Optics
A Versatile Tiling Light Sheet Microscope for Imaging of Cleared Tissues
Chen Yanlu, Li Xiaoliang, Zhang Dongdong, Wang Chunhui, Feng Ruili, Li Xuzhao, Wen Yao, Xu Hao, Zhang Xinyi Shirley, Yang Xiao, Chen Yongyi, Feng Yi, Zhou Bo, Chen Bi-Chang, Lei Kai, Cai Shang, Jia Jie-Min, Gao Liang
 [ DOI here ]    Year:2020-11
Cell Reports
Aging shifts mitochondrial dynamics toward fission to promote germline stem cell loss
Amartuvshin Oyundari, Lin Chi‐Hung, Hsu Shao‐Chun, Kao Shih‐Han, Chen Alvin, Tang Wei‐Chun, Chou Han‐Lin, Chang Dong‐Lin, Hsu Yen‐Yang, Hsiao Bai‐Shiou, Rastegari Elham, Lin Kun‐Yang, Wang Yu‐Ting, Yao Chi‐Kuang, Chen Guang‐Chao, Chen Bi‐Chang, Hsu Hwei‐Jan
 [ DOI here ]    Year:2020-07
Aging Cell
Simultaneous Single-Particle Tracking and Dynamic pH Sensing Reveal Lysosome-Targetable Mesoporous Silica Nanoparticle Pathways
Zhang Rong-Lin, Pratiwi Feby Wijaya, Chen Bi-Chang, Chen Peilin, Wu Si-Han, Mou Chung-Yuan
 [ DOI here ]    Year:2020-07
ACS Applied Materials & Interfaces
SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network
Chakraborty Archan, Lin Wei-Cheng, Lin Yu-Tsun, Huang Kuang-Jing, Wang Pei-Yu, Chang Ian Yi-Feng, Wang Hsiang-Iu, Ma Kung-Ting, Wang Chun-Yen, Huang Xuan-Rong, Lee Yen-Hsien, Chen Bi-Chang, Hsieh Ya-Ju, Chien Kun-Yi, Lin Tzu-Yang, Liu Ji-Long, Sung Li-Ying, Yu Jau-Song, Chang Yu-Sun, Pai Li-Mei
 [ DOI here ]    Year:2020-05
Journal of Cell Science
PIP3 depletion rescues myoblast fusion defects in human rhabdomyosarcoma cells
Lian Yen-Ling, Chen Kuan-Wei, Chou Yu-Ting, Ke Ting-Ling, Chen Bi-Chang, Lin Yu-Chun, Chen Linyi
 [ DOI here ]    Year:2020-04
Journal of Cell Science
Rapid high resolution 3D imaging of expanded biological specimens with lattice light sheet microscopy
Tsai Yun-Chi, Tang Wei-Chun, Low Christine Siok Lan, Liu Yen-Ting, Wu Jyun-Sian, Lee Po-Yi, Chen Lindsay Quinn, Lin Yi-Ling, Kanchanawong Pakorn, Gao Liang, (Chen Bi-Chang*)
 [ DOI here ]    Year:2020-03
Two-beam interference lattice lightsheet for structured illumination microscopy
Chang Bo-Jui, Tang Wei-Chun, Liu Yen-Ting, Tsai Yun-Chi, Tsao Chieh, Chen Peilin, Chen Bi-Chang
 [ DOI here ]    Year:2019-11
Journal of Physics D: Applied Physics
Aurora B functions at the apical surface after specialized cytokinesis during morphogenesis in C. elegans
Bai Xiaofei, Melesse Michael, Sorensen Turpin Christopher G., Sloan Dillon, Chen Chin-Yi, Wang Wen-Cheng, Lee Po-Yi, Simmons James R., Nebenfuehr Benjamin, Mitchell Diana, Klebanow Lindsey R., Mattson Nicholas, Betzig Eric, Chen Bi-Chang, Cheerambathur Dhanya, Bembenek Joshua N.
 [ DOI here ]    Year:2019
Lattice light sheet microscopy using tiling lattice light sheets
Gao Liang, Tang Wei-Chun, Tsai Yun-Chi, (Chen Bi-Chang*)
 [ DOI here ]    Year:2019
Optics Express
Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging
Lu Chieh-Han, Tang Wei-Chun, Liu Yen-Ting, Chang Shu-Wei, Wu Frances Camille M., Chen Chin-Yi, Tsai Yun-Chi, Yang Shun-Min, Kuo Chiung-Wen, Okada Yasushi, Hwu Yeu-Kuang, Chen Peilin, (Chen Bi-Chang*)
 [ DOI here ]    Year:2019
Communications Biology
Rapid single-wavelength lightsheet localization microscopy for clarified tissue
Chu Li-An, Lu Chieh-Han, Yang Shun-Min, Liu Yen-Ting, Feng Kuan-Lin, Tsai Yun-Chi, Chang Wei-Kun, Wang Wen-Cheng, Chang Shu-Wei, Chen Peilin, Lee Ting-Kuo, Hwu Yeu-Kuang, Chiang Ann-Shyn, (Chen Bi-Chang*)
 [ DOI here ]    Year:2019
Nature Communications
Recent advances in the use of fluorescent nanoparticles for bioimaging
Pratiwi Feby Wijaya, Kuo Chiung Wen, (Chen Bi-Chang), Chen Peilin
 [ DOI here ]    Year:2019
The Applications of Lattice Light-sheet Microscopy for Functional Volumetric Imaging of Hippocampal Neurons in a Three-Dimensional Culture System
Chen , Liu , Lu , Lee , Tsai , Wu , Chen , (B-C Chen*)
 [ DOI here ]    Year:2019
Adherens junction-associated pores mediate the intercellular transport of endosomes and cytoplasmic proteins
Yang Wen-Ting, Li Yu-Chiao, Lin Chiao-Ming, Tang Wei-Chun, Chen Bi-Chang, Suzuki Emiko, Hsu Jui-Chou
 [ DOI here ]    Year:2018-11
Biochemical and Biophysical Research Communications
Recent Progress in Light Sheet Microscopy for Biological Applications
Chatterjee Krishnendu, Pratiwi Feby Wijaya, Wu Frances Camille M., Chen Peilin, Chen Bi-Chang
 [ DOI here ]    Year:2018-06
Applied Spectroscopy
Glass-based microchip with finger electrodes for the formation, collection, and biointeractions of liposomes
Chiang Yuying
 [ DOI here ]    Year:2018-04
Journal of Micro\/Nanolithography, MEMS, and MOEMS
Actin-based protrusions of migrating neutrophils are intrinsically lamellar and facilitate direction changes
Fritz-Laylin Lillian K, Riel-Mehan Megan, Chen Bi-Chang, Lord Samuel J, Goddard Thomas D, Ferrin Thomas E, Nicholson-Dykstra Susan M, Higgs Henry, Johnson Graham T, Betzig Eric, Mullins R Dyche
 [ DOI here ]    Year:2017-09
Visualizing dynamic microvillar search and stabilization during ligand detection by T cells
Cai En, Marchuk Kyle, Beemiller Peter, Beppler Casey, Rubashkin Matthew G., Weaver Valerie M., Gérard Audrey, Liu Tsung-Li, (Chen Bi-Chang), Betzig Eric, Bartumeus Frederic, Krummel Matthew F.
 [ DOI here ]    Year:2017
A plasma membrane template for macropinocytic cups
Veltman, D. M.; Williams, T. D.; Bloomfield, G; Chen, B.-C.; Betzig, E.; Insall R.H. and Kay, R. R.,
 [ DOI here ]    Year:2016-12
V-1 Regulates Capping Protein Activity in Vivo
Jung, G; Alexander, C. J.; Wu, X.; Piszczek, G; Chen, B.-C.; Betzig, E. and Hammer, J. A.
 [ DOI here ]    Year:2016-10
Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy
Aguet, F.; Upadhyayula, S.; Gaudin, R.; Chou, Y-Y, Cocucci,E; He, H; Chen,B.-C.; Mosaliganti, K; Pasham, M; Skillern, W; Legant, W.; Liu,T.-L.; Findlay, G; Marino, E; Danuser,G; Megason, S; Betzig, E and Kirchhausen, T,
 [ DOI here ]    Year:2016-08
Molecular Biology of the Cell
Imaging Live at High Spatiotemporal Resolution for Lab-on-a-Chip Applications
Chin, L.; Lee, C.-H.; Chen, B.-C*.
 [ DOI here ]    Year:2016-04
Histone H3 Threonine Phosphorylation Regulates Asymmetric Histone Inheritance in the Drosophila Male Germline.
Xie, J.; Wooten, M.; Tran, V.; (Chen, B.-C.); Pozmanter, C.; Simbolon, C.; Betzig, E.; Chen, X.,
 [ DOI here ]    Year:2015-11
Three-dimensional tracking of plus-tips by lattice light-sheet microscopy permits the quantification of microtubule growth trajectories within the mitotic apparatus,
Yamashita, N.; Morita, M.; Legant, W. R.; (Chen, B.-C.); Betzig, E.; Yokoto, H.; Mimori-Kiyosue, Y.,
 [ DOI here ]    Year:2015-11
Vinculin is required for cell polarization, migration, and ECM-remodeling in 3D collagen.
Thievessen, I.; Fakhri, N.; Steinwachs, J.; Kraus, V.; Mclsaac R. S.; Gao, L.; (Chen, B.-C.); Baird, M. A.; Davidson, M. W.; Betzig, E.; Oldenbourg, R.; Waterman, C.M.; Fabry, B.,
 [ DOI here ]    Year:2015-11
Extended resolution structured illumination imaging of endocytic and cytoskeletal dynamics
Li, D.; Shao, L.; (Chen, B.-C.); Zhang, X.; Zhang, M.; Moses, B.; Milkie, D. E.; Beach, J. R.; Hammer III J. A.; Pasham M.; Kirchhausen, T.; Baird M. A.; Davidson, M. W.; Xu, P.; Betzig, E.,
 [ DOI here ]    Year:2015-08
Novel transport function of adherens junction revealed by live imaging in Drosophila
Li, Y.-C.; Yang, W.-T.; Cheng, L.-C.; Lin, Y.-H.; Ho, Y.-H.; Lin, P.-Y.; (Chen, B.-C.); Rickoll, W. L.; Hsu, J.-C.,
 [ DOI here ]    Year:2015-08
Actin depletion initiates events leading to granule secretion at the immunological synapse.
Ritter, A. T.; Asano, Y.; Stinchcombe J. C.; Dieckmann, N. M. G.; (Chen. B.-C.); Schuyler G. C.; Legant, W.; Gao, L.; Davidson, M. W.; Betzig, E.; Lippincott-Schwartz, J; Griffiths G. M.,
 [ DOI here ]    Year:2015-05
3D Imaging of Sox2 Enhancer Clusters in Embryonic Stem Cells
Liu, Z.; Legant, W.; (Chen, B.-C.); Li, L.; GrimmMilkie, J. B., Lavis, L. D.; Betzig, E.; Tjian, R,
 [ DOI here ]    Year:2014-12
Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically-disordered proteins in C. elegans
Wang, J. T.; Smith, J.; (Chen, B.-C.); Schmidt, H.; Rasoloson, D.; Paix, A.; Lambrus, B. G; Calidas, D.; Betzig, E.; Seydoux, G.,
 [ DOI here ]    Year:2014-12
Lattice light sheet microscopy-Imaging molecules to embryos at high spatiotemporal resolution.
(Chen, B.-C.); Legant, W.; Wang, K; Shao, L.; Milkie, D. E.; Davidson, M. W.; Janetopoulos, C.; Wu, X. S.; Hammer III, J. A.; Liu, Z.; English, B. P.; Mimori-Kiyosue, Y.; Ritter, A.; Lippincott-Schwartz, J; Fritz-Laylin, L.; Mullins, R. D.; Mitchell, D.; Bembenek, J. M.; Reyman, A-C.; Bohme, R.; Grill, S. W.; Wang, J.; Seydoux, G.; Tulu, U. S.; Kiehart, D. P.; Betzig, E.,
 [ DOI here ]    Year:2014-10
3D Live Fluorescence Imaging of Cellular Dynamics Using Bessel Beam Plane Illumination Microsocpy.
Gao, L. ; Shao, L.; (Chen, B.-C.); Betzig, E.
 [ DOI here ]    Year:2014-04
Nature Protocols
Single-molecule Dynamics of Enhanceosome Assembly in Embryonic Stem Cells
Chen, J.; Li, L.; Zhang, Z.; (Chen, B.-C.); Revyakin, A.; Hajj, B.; Legant, W.; Dahan, M.; Lionnet, T.; Betzig, E.; Tjian, R.; Liu, Z.,
 [ DOI here ]    Year:2014-03
A Localized Wnt Signal Orients Asymmetric Stem Cell Divison in Vitro
Habib, S. J.; (Chen, B.-C.); Tsai, F.-C.; Anastassiadis, K.; Meyer, T.; Betzig, E.;Nusse R.
 [ DOI here ]    Year:2013-03