李超煌

Chau-Hwang Lee

Education

  • 1993-1997 Ph.D. in Electrical Engineering, National Taiwan University.
  • 1992-1993 M.S. student in Graduate Institute of Electro-Optical Engineering, National Taiwan University.
  • 1988-1992 B.S. in Electrical Engineering, National Taiwan University.
  • 1985-1988 Taipei Municipal Chien Kuo Senior High School.

Research Fields

  • Cell-cell and cell-microenvironment interactions.
  • Super resolution far-field optical microscopy.
  • Differential sectioning microscopy.
  • Mechanical and electrical characteristics of bio-membranes.
  • Laser-assisted micro/nano fabrication.

Experience

  • Deputy Executive Secretary (2016-present), Central Academic Advisory Committee, Academia Sinica.
  • Joint-appointment Professor (2014-present), Professor and Chairman (2011-2014), Institute of Biophotonics, National Yang-Ming University.
  • 2012 Academia Sinica Research Award for Junior Research Investigators.
  • 2010 Dr. Ta-You Wu Memorial Award, National Science Council, Taiwan.
  • Research Fellow, Research Center for Applied Sciences, Academia Sinica (2010–present).
  • Joint-appointment Assistant and Associate Professor, Institute of Biophotonics, National Yang-Ming University (2003–2011).
  • Assistant to Associate Research Fellow, Research Center for Applied Sciences, Academia Sinica (2000–2010).
  • Postdoctoral Research Associate, Institute of Atomic and Molecular Sciences, Academia Sinica (1997–1999).
  • Excellent Ph.D. Thesis Award of the R.O.C. Optical Engineering Society (1997).

Patents:

Height measurement by correlating intensity with position of scanning object along optical axis of a structured illumination microscope. US Patent 8,705,043, ROC (Taiwan) Patent I438392.
Optical Sectioning Microcopy, US Patent 8,019,136, Japan Patent 5085608, ROC (Taiwan) Patent I414818.
Modulation Differential Confocal Microscopy, ROC (Taiwan) Patent I329207.
Method for Characterizing Transparent Thin-films Using Differential Optical Sectioning Interference Microscopy, US Patent 7,545,510, ROC (Taiwan) Patent I328676.
Differential Confocal Microscopy, ROC (Taiwan) Patent 082212, US Patent 5,804,813.

Publications

A 3D culture system for evaluating the combined effects of cisplatin and anti-fibrotic drugs on the growth and invasion of lung cancer cells co-cultured with fibroblasts
H.-J. Pan, C.-W. Lee, L.-Y. Wu, H.-H. Hsu, Y.-C. Tung, W.-Y. Liao*, and (C.-H. Lee)*
 [ DOI here ]    Year:2023-03
APL Bioengineering
Neurite growth induced by red light-caused intracellular reactive oxygen species production through cytochrome c oxidase activation
Y.-C. Kao, G.-Y. Lin, J.-Y. Cheng, and (C.-H. Lee)*
 [ DOI here ]    Year:2023-02
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Effects of the media conditioned by various macrophage subtypes derived from THP-1 cells on tunneling nanotube formation in pancreatic cancer cells
C.-W. Lee, C.-C. Kuo, C.-J. Liang, H.-J. Pan, C.-N. Shen*, and (C.-H. Lee)*
    Year:2022-07
BMC Molecular and Cell Biology
Hydrostatic pressure promotes migration and filamin-A activation in fibroblasts with increased p38 phosphorylation and TGF-beta production
Y.-C. Kao, Z.-H. Chen, W.-Y. Wang, (C.-H. Lee)*, and P.-L. Kuo*
    Year:2021-07
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Autophagy in fibroblasts induced by cigarette smoke extract promotes invasion in lung cancer cells
H.-H. Hou, H.-J. Pan, W.-Y. Liao, (C.-H. Lee)*, and C.-J. Yu*
    Year:2020-11
INTERNATIONAL JOURNAL OF CANCER
Neurite regrowth stimulation by a red-light spot focused on the neuronal cell soma following blue light-induced retraction
Y.-C. Kao, Y.-C. Liao, P.-L. Cheng, and (C.-H. Lee)*
 [ DOI here ]    Year:2019-12
SCIENTIFIC REPORTS
Emerging roles of air gases in lipid bilayers
C.-W. Lee, Y.-L. Chiang, J.-T. Liu, Y.-X. Chen, (C.-H. Lee)*, Y.-L. Chen*, and I.-S. Hwang*
    Year:2018-09
SMALL
Substrate properties modulate cell membrane roughness by way of actin filaments
C.-H. Chang, H.-H. Lee*, (C.-H. Lee)*
    Year:2017-08
SCIENTIFIC REPORTS
The affinity of elongated membrane-tethered ligands determines potency of T cell receptor triggering
B.-M. Chen, M. A. Al-Aghbar, C.-H. Lee, T.-C. Chang, Y.-C. Su, Y.-C. Li, S.-E. Chang, C.-C. Chen, T.-H. Chung, Y.-J. Liao, (C.-H. Lee), S. Roffler*
    Year:2017-07
FRONTIERS IN IMMUNOLOGY
Mechanoprofiling on membranes of living cells with atomic force microscopy and optical nano-profilometry
C.-W. Lee, C.-C. Wang, (C.-H. Lee)*
    Year:2017-06
Advances in Physics: X
Elevated hydrostatic pressure enhances the motility and enlarges the size of the lung cancer cells through aquaporin upregulation mediated by caveolin-1 and ERK1/2 signaling
Y.-C. Kao, J.-R. Jheng, H.-J. Pan, W.-Y. Liao, (C.-H. Lee)*, P.-L. Kuo*
    Year:2017-02
ONCOGENE
Imaging live cells at high spatiotemporal resolution for lab-on-a-chip applications
L. K. Chin, (C.-H. Lee), and B.-C. Chen*
    Year:2016-05
LAB ON A CHIP
Cell refractive index for cell biology and disease diagnosis: past, present and future
P. Y. Liu, L. K. Chin*, W. Ser*, H. F. Chen, C.-M. Hsieh, (C.-H. Lee), K.-B. Sung, T. C. Ayi, P. H. Yap, B. Liedberg, K. Wang, T. Bourouinaj, Y. Leprince-Wan*
    Year:2016-02
LAB ON A CHIP
Drug testing and flow cytometry analysis on a large number of uniform sized tumor spheroids using a microfluidic device
B. Patra, C.-C. Peng, W.-H. Liao, (C.-H. Lee), Y.-C. Tung*
    Year:2016-02
Scientific Reports
Membrane roughness as a sensitive parameter reflecting the status of neuronal cells in response to chemical and nanoparticle treatments
C.-W. Lee, L.-L. Jang, H.-J. Pan, Y.-R. Chen, C.-C. Chen, (C.-H. Lee)*
    Year:2016-01
Journal of Nanobiotechnology
High aspect ratio nanoimprinted grooves of poly(lactic-co-glycolic acid) control the length and direction of retraction fibers during fibroblast cell division
Y.-H. Su, P.-C. Chiang, L.-J. Cheng, (C.-H. Lee), N. S. Swami*, C.-F. Chou*
    Year:2015-12
Biointerphases
Directional migration of cancer cells induced by a blue light intensity gradient
C.-C. Lan, E. Y. Lu, H.-J. Pan, (C.-H. Lee)*
    Year:2015-07
Biomedical Optics Express
Effects of hydraulic pressure on cardiomyoblasts in a microfluidic device
Y.-F. Hsiao, H.-J. Pan, Y.-C. Tung, C.-C. Chen*, (C.-H. Lee)*
    Year:2015-04
Biomicrofluidics
Heparin cofactor II enhances cell motility and promotes metastasis in non-small-cell lung cancer
W.-Y. Liao, C.-C. Ho, H.-H. Hou, T.-H. Hsu, M.-F. Tsai, K.-Y. Chen, H.-Y. Chen, Y.-C. Lee, C.-J. Yu, (C.-H. Lee)*, P.-C. Yang*
    Year:2015-01
JOURNAL OF PATHOLOGY
Migration and vascular lumen formation of endothelial cells in cancer cell spheroids of various sizes
B. Patra, Y.-S. Peng, C.-C. Peng, W.-H. Liao, Y.-A. Chen, K.-H. Lin, Y.-C. Tung*, (C.-H. Lee)*
    Year:2014-09
Biomicrofluidics
Modulating chemotaxis of lung cancer cells by using electric fields in a microfluidic device
Y.-C. Kao, M.-H. Hsieh, C.-C. Liu, H.-J. Pan, W.-Y. Liao, J.-Y. Cheng, P.-L. Kuo,* (C.-H. Lee)*
    Year:2014-04
Biomicrofluidics
Substrate stiffness regulates filopodial activities in lung cancer cells
Y.-R. Liou, W. Torng, Y.-C. Kao, K.-B. Sung, (C.-H. Lee), P.-L. Kuo*
    Year:2014-02
PLoS One
Single-virus tracking approach to reveal the interaction of Dengue virus with autophagy during the early stage of infection
L.-W. Chu, Y.-L. Huang, J.-H. Lee, L.-Y. Huang, W.-J. Chen, Y.-H. Lin, J.-Y. Chen, R. Xiang, (C.-H. Lee), Y.-H. Ping*
    Year:2014-01
JOURNAL OF BIOMEDICAL OPTICS
Using optical profilometry to characterize cell membrane roughness influenced by Amyloid-beta 42 aggregates and electric fields
H.-J. Pan, R.-L. Wang, J.-L. Xiao, Y.-J. Chang, J.-Y. Cheng, Y.-R. Chen, (C.-H. Lee)*
    Year:2014-01
JOURNAL OF BIOMEDICAL OPTICS
A microfluidic device for uniform-sized cell spheroids formation, culture, harvesting and flow cytometry analysis
B. Patra, Y.-H. Chen, C.-C. Peng, S.-C. Lin, (C.-H. Lee),* Y.-C. Tung*
    Year:2013-09
Biomicrofluidics
Evaluation of EGFR and RTK signaling in the electrotaxis of lung adenocarcinoma cells under direct-current electric field stimulation
H.-F. Tsai, C.-W. Huang, H.-F. Chang, J. J. W. Chen, (C.-H. Lee), J.-Y. Cheng*
    Year:2013-08
PLoS One
Guiding the migration of adherent cells by using optical micropatterns
J.-L. Xiao, D.-H. Lu, (C.-H. Lee)*
    Year:2013-03
APPLIED PHYSICS LETTERS
Interaction between lung cancer cell and myofibroblast influenced by cyclic tensile strain
J.-W. Huang, H.-J. Pan, W.-Y. Yao, Y.-W. Tsao, W.-Y. Liao, C.-W. Wu, Y.-C. Tung*, (C.-H. Lee)*
    Year:2013-03
LAB ON A CHIP
Fabrication and modification of dual-faced nano-mushrooms for tri-functional cell theranostics: SERS/fluorescence signaling, protein targeting, and drug delivering
H.-Y. Hsieh, T.-W. Huang, J.-L. Xiao, C.-S. Yang, C.-C. Chang, C.-C. Chu, L.-W. Lo, S.-H. Wang, P.-C. Wang, C.-C. Chieng, (C.-H. Lee)*, F.-G. Tseng*
    Year:2012-09
JOURNAL OF MATERIALS CHEMISTRY
Optically micropatterned culture of adherent cells
J.-L. Xiao, H.-J. Pan, (C.-H. Lee)*
    Year:2012-07
JOURNAL OF BIOMEDICAL OPTICS
The migration speed of cancer cells influenced by macrophages and myofibroblasts co-cultured in a microfluidic chip
T.-H. Hsu, Y.-L. Kao, W.-L. Lin, J.-L. Xiao, P.-L. Kuo, C.-W. Wu, W.-Y. Liao, (C.-H. Lee*)
    Year:2012-02
Integrative Biology
Au-coated polystyrene nanoparticles with HAR nanocorrugations via surface carboxylation shielded anisotropic etching for significant SERS signal enhancement
H.-Y. Hsieh, J.-L. Xiao, (C.-H. Lee), T.-W. Huang, C.-S. Yang, P.-C. Wang*, and F.-G. Tseng*
    Year:2011-08
Journal of Physical Chemistry C
The ability of LCRMP-1 to promote cancer invasion by enhancing filopodia formation is antagonized by CRMP-1
S.-H. Pan, Y.-C. Chao, P.-F. Hung, H.-Y. Chen, S.-C. Yang, Y.-L. Chang, C.-T. Wu, C.-C. Chang, W.-L. Wang, W.-K. Chan, Y.-Y. Wu, T.-F. Che, L.-K. Wang, C.-Y. Lin, Y.-C. Lee, M.-L. Kuo, (C.-H. Lee), J. J. W. Chen, T.-M. Hong, P.-C. Yang*
    Year:2011-08
JOURNAL OF CLINICAL INVESTIGATION
Measurement of membrane rigidity on trapped unilamellar phospholipid vesicles by using differential confocal microscopy
T.-H. Liu, J.-L. Xiao, (C.-H. Lee), J.-Y. Lin*
    Year:2011-07
APPLIED OPTICS
Three-dimensional tracking and temporal analysis of liposomal transport in live cells using bright-field imaging
F.-C. Tsai, L.-A. Tai, Y.-J. Wang, J.-L. Xiao, T.-H. Hsu, C.-S. Yang, (C.-H. Lee)*
    Year:2011-06
MICROSCOPY RESEARCH AND TECHNIQUE
Analysis of the paracrine loop between cancer cells and fibroblasts using a microfluidic chip
T.-H. Hsu, J.-L. Xiao, Y.-W. Tsao, Y.-L. Kao, S.-H. Huang, W.-Y. Liao, (C.-H. Lee)*
    Year:2011-05
LAB ON A CHIP
Asymmetric cancer-cell filopodium growth induced by electric-fields in a microfluidic culture chip
C.-C. Wang, Y.-C. Kao, P.-Y. Chi, C.-W. Huang, J.-Y. Lin, C.-F. Chou, J.-Y. Cheng*, (C.-H. Lee)*
    Year:2011-02
LAB ON A CHIP
Motion of cancer-cell lamellipodia perturbed by laser light of two wavelengths
J.-L. Xiao, T.-H. Hsu, P.-Y. Hsu, W.-J. Yang, P.-L. Kuo, (C.-H. Lee)*
    Year:2010-11
APPLIED PHYSICS LETTERS
Three-dimensional characterization of active membrane waves on living ells
Chien-Hong Chen, Feng-Ching Tsai, Chun-Chieh Wang, (Chau-Hwang Lee)*
    Year:2009-12
PHYSICAL REVIEW LETTERS
Three-dimensional nanoparticle tracking and simultaneously membrane profiling during endocytosis of living cells
Chun-Chieh Wang, Chia-Pin Liang, (Chau-Hwang Lee)*
    Year:2009-11
APPLIED PHYSICS LETTERS
Wide-field optical nano-profilometry using structured illumination
Chun-Chieh Wang, Kuang-Li Lee, (Chau-Hwang Lee)*
    Year:2009-11
OPTICS LETTERS
Label-free quantification of asymmetric cancer-cell filopodium activities in a multi-gradient chip
T.-H. Hsu, M.-H. Yen, W.-Y. Liao, J.-Y. Cheng*, and (C.-H. Lee)*
    Year:2009-03
LAB ON A CHIP
Wide-field super-resolution optical sectioning microscopy using a single spatial light modulator
Jiunn-Yuan Lin, Ru-Ping Huang, Pei-Shui Tsai, and (Chau-Hwang Lee)*
    Year:2009-01
JOURNAL OF OPTICS A-PURE AND APPLIED OPTICS
Cell membrane deformations under magnetic force modulation characterized by optical tracking and profilometry
Chun-Chieh Wang, Hung-Jhang Jian, Chih-Wei Wu, and (Chau-Hwang Lee)*
    Year:2008-08
MICROSCOPY RESEARCH AND TECHNIQUE
Internalization of nanoparticles on cell membranes observed by non-interferometric wide-field optical profilometry
C.-C. Wang, C.-W. Lee, C.-Y. Huang, J.-Y. Lin, P.-K. Wei, and (C.-H. Lee)*
    Year:2008-05
APPLIED OPTICS
Transparent thin-film characterization by using differential optical sectioning interference microscopy
Chun-Chieh Wang, Jiunn-Yuan Lin, Hung-Jhang Jian, and (Chau-Hwang Lee)*
    Year:2007-10
APPLIED OPTICS
Fabrication of high-aspect-ratio silicon nanopillar arrays with the conventional reactive ion etching technique
Yu-Fen Chang, Qiong-Ru Chou, Jiunn-Yuan Lin, and (Chau-Hwang Lee)*
    Year:2007-02
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
Dynamics of cancer cell filopodia characterized by super-resolution bright-field optical microscopy
T.-H. Hsu, W.-Y. Liao, P.-C. Yang, C.-C. Wang, J.-L. Xiao, and (C.-H. Lee)*
    Year:2007-01
OPTICS EXPRESS
Dynamics of cell membranes and the underlying cytoskeletons observed by non-interferometric wide-field optical profilometry and fluorescence microscopy
Chun-Chieh Wang, Jiunn-Yuan Lin, Hsiao-Chao Chen, and (Chau-Hwang Lee)*
    Year:2006-10
OPTICS LETTERS
Membrane ripples of a living cell measured by non-interferometric wide-field optical profilometry
Chun-Chieh Wang, Junn-Yuan Lin, and (Chau-Hwang Lee)*
    Year:2005-12
OPTICS EXPRESS
Optical measurement of the deformation of giant lipid vesicles driven by a micropipette electrode
(Chau-Hwang Lee)*, Yu-Fen Chang, Chia-Hsuan Tsai and Po-Hsiang Wang
    Year:2005-08
LANGMUIR
Fabricating high-aspect-ratio sub-diffraction-limit structures on silicon with two-photon photopolymerization and reactive ion etching
(Chau-Hwang Lee)*, Ting-Wei Chang, Kuang-Li Lee, Jiunn-Yuan Lin, and Jyhpyng Wang
    Year:2004-12
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
Super-resolution bright-field optical microscopy based on nanometer topographic contrast
Shu-Wei Huang, Hong-Yao Mong, and (Chau-Hwang Lee)*
    Year:2004-11
MICROSCOPY RESEARCH AND TECHNIQUE
High-brightness soft x-ray generation under optimized laser-cluster interaction
J.-Y. Lin, H.-H. Chu, M.-Y. Shen, Y.-F. Xiao, (C.-H. Lee)*, S.-Y. Chen, and J. Wang
    Year:2004-02
OPTICS COMMUNICATIONS
Sub-diffraction-limit imaging based on the topographic contrast of differential confocal microscopy
(Chau-Hwang Lee)*, Hui-Yu Chiang, and Hong-Yao Mong
    Year:2003-10
OPTICS LETTERS
Non-interferometric wide-field optical profilometry with nanometer depth resolution
(Chau-Hwang Lee)*, Hong-Yao Mong, and Wan-Chen Lin
    Year:2002-10
OPTICS LETTERS
Using differential confocal microscopy to detect the phase transition of lipid vesicle membranes
(Chau-Hwang Lee)*, Wan-Chen Lin, and Jyhpyng Wang
    Year:2001-10
OPTICAL ENGINEERING
All-optical measurements of the bending rigidity of lipid-vesicle membranes across structural phase transitions\t
(Chau-Hwang Lee)*, Wan-Chen Lin, and Jyhpyng Wang
    Year:2001-08
PHYSICAL REVIEW E
Femtosecond self-focusing dynamics measured by three-dimensional phase-retrieval cross correlation
Tai-Wei Yau, (Chau-Hwang Lee), and Jyhpyng Wang*
    Year:2000-09
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS
Deconvolution of local surface response from topography in nanometer profilometry with a dual-scan method
Chao-Wei Tsai, (Chau-Hwang Lee), and Jyhpyng Wang*
    Year:1999-12
OPTICS LETTERS
Optical measurement of the viscoelastic and biochemical responses of living cells to mechanical perturbation
(Chau-Hwang Lee), Chinlin Guo, and Jyhpyng Wang*
    Year:1998-02
OPTICS LETTERS
Dynamic model of multipass ultrashort-pulse laser amplifiers and its application
Yi-Yuh Hwang, (Chau-Hwang Lee), and Jyhpyng Wang
    Year:1997-10
APPLIED OPTICS
Noninterferometric differential confocal microscopy with 2-nm depth resolution
(Chau-Hwang Lee) and Jyhpyng Wang
    Year:1997-02
OPTICS COMMUNICATIONS
Femtosecond transform-limited Kerr-lens mode-locked dye lasers
(Chau-Hwang Lee), Jyhpyng Wang*, and Yuh-Fwu Chou
    Year:1996-10
OPTICAL AND QUANTUM ELECTRONICS
Characteristics of a femtosecond transform-limited Kerr-lens mode-locked dye laser
Yuh-Fwu Chou, (Chau-Hwang Lee), and Jyhpyng Wang*
    Year:1994-07
OPTICS LETTERS