Bi-Chang Chen 陳壁彰
Associate Research Fellow

Ways to contact me:
Phone:02-2787-3133 or 2787-3152
Fax: 02-2787-3122
Address: Research Center for Applied Sciences, Academia Sinica
128 Sec. 2, Academia Rd., Nankang, Taipei 11529, Taiwan
Job Opening : At all levels



Research Fields:

  • Light Sheet Microscopy
  • Developing fast, low phototoxicity, multi-color, and 3D detection for fluorescent living specimens with subcellular resolution imaging tools
  • Applications ranging from single molecule tracking to high spatiotemporal imaging of subcellular dynamics within whole developing embryos.

Recent Publications:

  1. Wu, J.-L.; Kuan, I.-I.; Guo, J.-Y.; Hsu, W. C.; Tang, W.-C.; Chan, H.-J.; Chen, Y. J.; Chen, B.-C.; Wu, H.-C.; Liao, J. C.* "SARS-CoV-2 N protein mediates intercellular nucleic acid dispersion, a feature reduced in Omicron", iScience , 105995 (2023)
    [ DOI:10.1016/j.isci.2023.105995 ]
  2. Laiman, J.; Hsu, Y.-J.; Loh, J.; Tang, W.-C.; Chung, M.-C.; Liu, H.K.; Yang, W.-S.; Chen, B.-C.; Chuang, L.-M.; Chang, Y.-C.; Liu, Y.-W."GSK3α phosphorylates dynamin-2 to promote GLUT4 endocytosis in muscle cells", Journal of Cell Biology , 222 (2): e202102119 (2023)
    [ DOI:10.1083/jcb.202102119 ]
  3. Lu, C.-H.*; Huang, C.-Y.; Tian, X.; Chen, P.; Chen, B.-C.* "Large-scale expanded sample imaging with tiling lattice lightsheet microscopy", The International Journal of Biochemistry & Cell Biology , 154, 106340 (2023)
    [ DOI:10.1016/j.biocel.2022.106340 ]
  4. Tang, W.-C.; Liu, Y.-T.; Yeh, C.-H.; Lu, C.-H.; Tu, C.-H.; Lin, Y.-L.; Lin, Y. C.; Hsu, T.-L.; Gao, L.; Chang, S.-W.; Chen, P.*; Chen, B.-C.* "Optogenetic Manipulation of Cell Migration with High Spatiotemporal Resolution Using Lattice Lightsheet Microscopy", Communications Biology , 5, 879 (2022)
    [ DOI:10.1038/s42003-022-03835-6 ]
  5. Chen, C, Chang, T.-Y., Liang, S.-Y., Tsai,Y.-C., Chen, B.-C., Tu, H.-L., Cheng, P.-L.* "Neuronal paxillin and Drebrin mediate BDNF-induced force transduction and growth cone turning in a soft tissue-like environment.", Cell Reports , 40, 111188 (2022)
    [ DOI:10.1016/j.celrep.2022.111188 ]
  6. Wada, N.; Hsu, M.-T.; Tandon, K.; Hsiao, S. S.Y.; Chen, H.J.; Chen, Y. H.; Chiang, P.-W.; Yu, S.P.; Lu, C.-Y.; Chiou, Y.-J.; Tu, Y.-C.; Tian, X.; Chen, B.-C., Lee, D.-C.; Yamashiro, H.; Bourne, D.G.;Tang, S.-L.* "High-resolution spatial and genomic characterization of coral-associated microbial aggregates in the coral Stylophora pistillata", Science Advance , 8, eabo2431 (2022)
    [ DOI:10.1126/sciadv.abo2431 ]
  7. Lin, M.-J.; Lee, C.-M.; Hsu, W.-L.; Chen, B.-C., Lee, S.-J. "Macrophages break interneuromast cell quiescence by intervening in the inhibition of Schwann cells in the zebrafish lateral line", Frontiers in Cell and Developmental Biology, 10:907863 (2022)
    [ DOI:10.3389/fcell.2022.907863 ]
  8. Fan, Y.-J.*; Hsieh, H.-Y.; Huang, Y.-R.; Tsao, C.; Lee, C.-M.; Tahara,H.; Wu,Y.-C.; Sheen, H.-J.*; Chen, B.-C.*, "Development of a water refractive index-matched microneedle integrated into a light sheet microscopy system for continuous embryonic cell imaging", Lab on a chip, 22, 584-591 (2022)
    [ DOI:10.1039/D1LC00827G ]
  9. Lin, P.-Y.; Hwang, S.-P.; Lee, C.-H.*; Chen, B.-C.*, "Two-photon scanned light sheet fluorescence microscopy with axicon imaging for fast volumetric imaging", J Biomed Opt.,26(11):116503 (2021)
    [ DOI:10.1117/1.JBO.26.11.116503 ]
  10. Lin, M.-H.; Chen, J.-C.; Tian, X.; Lee, C.-M.; Yu,I-S.; Lo, Y.-F.; Uchida, S.; Huang, C.-L.; Chen, B.-C.*; Cheng, C.-J.*, "Impairment in renal medulla development underlies salt wasting in Clc-k2 channel deficiency", JCI Insight ,9:151039 (2021), selected as cover image
    [ DOI:10.1172/jci.insight ]
  11. Lee,C.-M.#; Tian, X.#; Tsao, C.#; Chen, P.; Huang T.-N.; Hsueh,Y.-P.; Chen, B.-C.*, "Macro Photography with Lightsheet Illumination Enables Whole Expanded Brain Imaging with Single-cell Resolution", Discoveries Journals , Jul-Sep, 9(3):e133 (2021)
    [ DOI:10.15190/d.2021.12 ]
  12. Chu, L.-A.*; Chang, S.-W.; Tang, W.-C.; Tseng, Y.-T.; Chen, P.; Chen, B.-C.*, "5D super-resolution imaging for a live cell nucleus", Current Opinion in Genetics & Development , 67, 77 (2021)
    [ DOI:10.1016/j.gde.2020.11.005 ]
  13. Fan, Y.-J.*; Hsieh, H.-Y.; Tsai, S.-F.; Wu, C.-H.; Lee, C.-M.; Liu, Y.-T.; Lu, C.-H.; Chang, S.-W.*; Chen, B.-C.*, "Microfluidic channel integrated with a lattice lightsheet microscopic system for continuous cell imaging", Lab on a chip, 21, 344 (2021)
    [ DOI:10.1039/d0lc01009j ]
  14. Chen, Y.; Li, X.; Zhang, D.; Wang, C.; Feng, R.; Li, X.; Wen, Y.; Xu, H.; Zhang, X. S.; Chen, Y.; Feng. Y.; Zhou, B.; Chen, B.-C.; Chen, P.; Lei, K.; Cai, S.; Jia, J.-M.; Gao, L. "A Versatile Tiling Light Sheet Microscope for Imaging of Cleared Tissues", Cell Reports, 33, 108349 (2020)
    [ DOI:10.1016/j.celrep.2020.108349 ]
  15. Chang, R.-L., Pratiwi, F. W., Chen, B.-C., Chen, P., Wu, S.-H. , Mou, C.-Y. "Simultaneous Single-particle Tracking and Dynamic pH Sensing Reveal Lysosome-targetable Mesoporous Silica Nanoparticles Pathways", ACS Applied Materials & Interfaces, 12, 42472 (2020)
    [ DOI:10.1021/acsami.0c07917 ]
  16. Amartuvshin, O., Lin, C.‐H., Hsu, S.‐C., Kao, S.‐H., Chen, A., Tang, W.‐C., Chou, H.‐L., Chang, D.‐L., Hsu, Y.‐Y., Hsiao, B.‐S.,Rastegari, E., Lin, K.‐Y., Wang, Y.‐T., Yao, C.‐K., Chen, G.‐C., Chen, B.-C., Hsu, H.‐J.“Aging shifts mitochondrial dynamics toward fission to promote germline stem cell loss”,Aging Cell, 19, e13191(2020)
    [ DOI:10.1111/acel.13191 ]
  17. Chakraborty, A. Lin, W.-C., Lin, Y.-T., Huang, K.-J., Wang,P.-Y., Chang, I. Y.-F., Wang, H.-I., Ma, K.-T., Wang, C.-Y., Huang, X.-R., Lee, Y.-H., Chen, B.-C., Hsieh, Y.-J., Chieh. K.-Y., Lin, T.-Y., Liu, J.-L, Sung, L.-Y., Yu, J.-S., Chang, Y.-S. and Pai, L.-M. "SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network", Journal of Cell Science, 133, jcs240200(2020)
    [ DOI:10.1242/jcs.240200 ]
  18. Lian, Y.-L., Chen, K.-W., Chou, Y.-T., Ke, T.-L., Chen, B.-C. , Lin, Y.-C., and Chen, L.“PIP3 depletion rescues myoblast fusion defects in human rhabdomyosarcoma cells”,Journal of Cell Science 133, jcs240325(2020)
    [ DOI:10.1242/jcs.240325 ]
  19. Chang, B.-J.; Tang, W.-C.; Liu, Y.-T.; Tsai, Y.-C.; Tsao, C.; Chen, P.; Chen, B.-C. “Two-beam interference lattice lightsheet for structured illumination microscopy” J. Phys. D: Appl. Phys. ,53, 044005(2020)
    [ DOI:10.1088/1361-6463/ab50e2 ]
  20. Tsai, Y.-C.; Tang, W.-C.; Low, C. S. L.; Liu, Y.-T.; Wu, J.-S.; Lee, P.-Y.; Lin, Y.-L.; Kanchanawong, P.; Gao, L, and Chen, B.-C. “Rapid High Resolution 3D Imaging of Expanded Biological Specimens with Lattice Lightsheet Microscopy”, Methods,174,11-19(2020)
    [ DOI:10.1016/j.ymeth.2019.04.006 ]
  21. Bai, X., Melesse M., Turpin, C. G. S., Sloan, D.; Chen, C.-Y.; Wang, W.-C.; Lee, P.-Y.; Simmons, J. R.; Nebenfuehr, B.; Mitchell M.; Klebanow, L. R.; Mattson, N.; Betzig, E.; Chen, B.-C. , Cheerambathur, D. and Bembenek, J. N. “Aurora B Functions at the Apical Surface after Specialized Cytokinesis during Morphogenesis in C.” elegans, Development , dev.181099(2019)
    [ DOI:10.1242/dev.181099 ]
  22. Chu, L.-A., Lu, C.-H., Yang, S.-M., Liu, Y-T, Feng, K.-L., Tsai, Y.-C., Chang, W.-K., Wang, W.-C., Chang, S.-W., Chen, P.; Lee, T.-K., Hwu, Y.-K., Chiang, A.-S.; and Chen, B.-C., "Rapid single-wavelength lightsheet localization microscopy for clarified tissue" Nature Communications, 10, 4762(2019)
    [ DOI:10.1038/s41467-019-12715-3 ]
  23. Pratiwi, F. W.; Kuo, C. W.; Chen, B.-C. and Chen, P., "Recent advances in the use of nanoparticles for bioimaging" Nanomedicine, 14, 1759-1769(2019)
    [ DOI:10.2217/nnm-2019-0105 ]
  24. Chen, C.-Y. ; Liu, Y.-T.; Lu, C.-H.; Lee, P.-Y., Tsai,Y.-C.; Wu, J.-S.; Chen, P. and Chen, B.-C., “The applications of lattice light-sheet microscopy for functional volumetric imaging of hippocampal neurons in a three-dimensional culture system”, Micromachine, 10, 599(2019)
    [ DOI:10.3390/mi10090599 ]
  25. Lu, C.-H.; Tang, W.-C.; Liu, Y.-T.; Wu, F. C. M.; Chen, C. Y.; Tsai, Y. C.; Yang, S.-M; Kuo, C.-W.; Okada, Y.; Hwu, Y.-K.; Chang, S.-W.; Chen, P. and Chen, B.-C., “Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging.” Communications Biology, 2, 177(2019)
    [ DOI:10.1038/s42003-019-0403-9 ]
  26. Gao, L.; Tang,W.-C.; Tsai, Y.-C.; Chen, B.-C. “Lattice light sheet microscopy using tiling lattice light sheets” Opt. Express, 27 1497-1506(2019)
    [ DOI:10.1364/OE.27.001497 ]
  27. Yang,W.-T.; Li,Y.-C.; Lin,C.-M.; Tang,W.-C.; Chen, B.-C.; Emiko Suzuki,; Hsu,J.-C., Adherens junction-associated pores mediate the intercellular transport of endosomes and cytoplasmic proteins,Biochemical and Biophysical Research Communications,505(2018)
    [ DOI:10.1016/j.bbrc.2018.09.129 ]
  28. Chatterjee,; Krishnendu,; Feby Wijaya Pratiwi,; Frances Camille M.; Chen,P.-L.; and Chen, B.-C., “Recent Progress in Light Sheet Microscopy for Biological Applications.” Applied Spectroscopy,72,1137–1169(2018)
    [ DOI:10.1177/0003702818778851 ]
  29. Chiang,Y.-Y.; Tsou,C.-F.; Chen, B.-C.; Chou,R.-H.; "Glass-based microchip with finger electrodes for the formation, collection, and biointeractions of liposomes," Journal of Micro/Nanolithography, MEMS, and MOEMS,17(2), 025001.(2018)
    [ DOI:10.1117/1.JMM.17.2.025001 ]
  30. Cai, E., K. Marchuk, P. Beemiller, C. Beppler, M. G. Rubashkin, V. M. Weaver, A. Gérard, Liu,T.-L.; Chen, B.-C.; E. Betzig, F. Bartumeus and M. F. Krummel. "Visualizing dynamic microvillar search and stabilization during ligand detection by T cells." Science 356(6338).(2017)
    [ DOI:10.1126/science.aal3118 ]
  31. Fritz-Laylin, L. K.; Riel-Mehan, M.; Chen, B.-C.; Lord, S. J.; Goddard T. D.; Ferrin, T. E.; Nicholson-Dykstra, S. M.; Higgs, H.; Johnson G.T.; Betzig, E,; Mullins, R. D. Actin-based protrusions of migrating neutrophils are intrinsically lamellar and facilitate direction changes, eLife, 6:e26990 (2017)
    [ DOI:10.7554/eLife.26990 ]
  32. Veltman, D. M.; Williams, T. D.; Bloomfield, G; Chen, B.-C.; Betzig, E.; Insall R.H. and Kay, R. R.,A plasma membrane template for macropinocytic cups, eLife, 5:e20085 (2016)
    [ DOI:10.7554/eLife.20085 ]
  33. Jung, G; Alexander, C. J.; Wu, X.; Piszczek, G; Chen, B.-C.; Betzig, E. and Hammer, J. A.,V-1 Regulates Capping Protein Activity in Vivo PNAS, 113, E6610–E6619 (2016)
    [ DOI:10.1073/pnas.1605350113 ]
  34. Aguet, F.; Upadhyayula, S.; Gaudin, R.; Chou, Y-Y, Cocucci,E; He, H; ,Chen,B.-C.; Mosaliganti, K; Pasham, M; Skillern, W; Legant, W.; Liu,T.-L.; Findlay, G; Marino, E; Danuser,G; Megason, S; Betzig, E and Kirchhausen, T, Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy Molecular Biology of the Cell, 27, 3418-3435 (2016)
    [ DOI:10.1091/mbc.E16-03-0164 ]
  35. Chin, L.; Lee, C.-H.; Chen, B.-C.. Imaging Live at High Spatiotemporal Resolution for Lab-on-a-Chip Applications, Lab on a Chip , 16, 2014-2024 (2016)

    [ DOI:10.1039/C5LC01556A ]
  36. Yamashita, N.; Morita, M.; Legant, W. R.; Chen, B.-C.; Betzig, E.; Yokoto, H.; Mimori-Kiyosue, Y., Three-dimensional tracking of plus-tips by lattice light-sheet microscopy permits the quantification of microtubule growth trajectories within the mitotic apparatus, J. Biomed. Opt., 20, 101206.(2015)
    [ DOI:10.1117/1.JBO.20.10.101206 ]
  37. Xie, J.; Wooten, M.; Tran, V.; Chen, B.-C.; Pozmanter, C.; Simbolon, C.; Betzig, E.; Chen, X., Histone H3 Threonine Phosphorylation Regulates Asymmetric Histone Inheritance in the Drosophila Male Germline. Cell, 163, 920-933, (2015)
    [ DOI:10.1016/j.cell.2015.10.002 ]
  38. Li, D.; Shao, L.;Chen, B.-C.; Zhang, X.; Zhang, M.; Moses, B.; Milkie, D. E.; Beach, J. R.; Hammer III J. A.; Pasham M.; Kirchhausen, T.; Baird M. A.; Davidson, M. W.; Xu, P.; Betzig, E., Extended resolution structured illumination imaging of endocytic and cytoskeletal dynamics. Science, 349, 6251 (2015)
    [ DOI:10.1126/science.aab3500 ]
  39. Thievessen, I.; Fakhri, N.; Steinwachs, J.; Kraus, V.; Mclsaac R. S.; Gao, L.;Chen, B.-C.; Baird, M. A.; Davidson, M. W.; Betzig, E.; Oldenbourg, R.; Waterman, C.M.; Fabry, B., Vinculin is required for cell polarization, migration, and ECM-remodeling in 3D collagen. FASEB J., pii:fj.14-268235 (2015)
    [ DOI:10.1096/fj.14-268235fj.14-268235 ]
  40. Li, Y.-C.; Yang, W.-T.; Cheng, L.-C.; Lin, Y.-H.; Ho, Y.-H.; Lin, P.-Y.;Chen, B.-C.; Rickoll, W. L.; Hsu, J.-C., Novel transport function of adherens junction revealed by live imaging in Drosophila. Biochem Biophys Res Commun., 463(4):686-92 (2015)
    [ DOI:10.1016/j.bbrc.2015.05.125. ]
  41. Kural, C.; Akatay, AA.; Gaudin, R.; Chen, B.-C.; Legant, WR.; Betzig, E.; Kirchhausen, T.,Asymmetric formation of coated pits on dorsal and ventral surfaces at the leading edges of motile cells and on protrusions of immobile cells.Molecular Biology of the Cell, 26,2044-2053(2015)
    [ DOI:10.1091/mbc.E15-01-0055 ]
  42. Ritter, A. T.; Asano, Y.; Stinchcombe J. C.; Dieckmann, N. M. G.; Chen, B.-C. ; Schuyler G. C.; Legant, W.; Gao, L.; Davidson, M. W.; Betzig, E.; Lippincott-Schwartz, J; Griffiths G. M., Actin depletion initiates events leading to granule secretion at the immunological synapse. Immunity, 42, 864-876 (2015)
    [ DOI:10.1016/j.immuni.2015.04.013 ]
  43. Wang, J. T.; Smith, J.; Chen, B.-C.; Schmidt, H.; Rasoloson, D.; Paix, A.; Lambrus, B. G; Calidas, D.; Betzig, E.; Seydoux, G., Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically-disordered proteins in C. elegans. eLife, DOI : 10.7554/eLife.04591 (2014).
    [ DOI:10.7554/eLife.04591 ]
  44. Liu, Z.; Legant, W.; Chen, B.-C.; Li, L.; GrimmMilkie, J. B., Lavis, L. D.; Betzig, E.; Tjian, R, 3D Imaging of Sox2 Enhancer Clusters in Embryonic Stem Cells. eLife, DOI : 10.7554/eLife.04236 (2014).
    [ DOI:10.7554/eLife.04236 ]
  45. Chen, B.-C.; Legant, W.; Wang, K; Shao, L.; Milkie, D. E.; Davidson, M. W.; Janetopoulos, C.; Wu, X. S.; Hammer III, J. A.; Liu, Z.; English, B. P.; Mimori-Kiyosue, Y.; Ritter, A.; Lippincott-Schwartz, J; Fritz-Laylin, L.; Mullins, R. D.; Mitchell, D.; Bembenek, J. M.; Reyman, A-C.; Bohme, R.; Grill, S. W.; Wang, J.; Seydoux, G.; Tulu, U. S.; Kiehart, D. P.; Betzig, E., Lattice Light Sheet Microscopy: Imaging Molecules to Embryos at High Spatiotemporal Resolution . Science, 346, 1257998/1-1257998/12 (2014).
    [ DOI:10.1126/science.1257998 ]
  46. Chen, J.; Li, L.; Zhang, Z.; Chen, B.-C.; Revyakin, A.; Hajj, B.; Legant, W.; Dahan, M.; Lionnet, T.; Betzig, E.; Tjian, R.; Liu, Z., Single-molecule Dynamics of Enhanceosome Assembly in Embryonic Stem Cells, Cell, 156, 1274-1285 (2014).
    [ DOI:10.1016/j.cell.2014.01.062 ]
  47. Gao, L. ; Shao, L.; Chen, B.-C.; Betzig, E., 3D Live Fluorescence Imaging of Cellular Dynamics Using Bessel Beam Plane Illumination Microsocpy. Nature Protocols , 9, 1083-1101 (2014). Selected for the cover article
    [ DOI:10.1038/nprot.2014.087 ]
  48. Habib, S. J.; Chen, B.-C.; Tsai, F.-C.; Anastassiadis, K.; Meyer, T.; Betzig, E.;Nusse R., A Localized Wnt Signal Orients Asymmetric Stem Cell Divison in Vitro. Science, 22, 1445-1448 (2013).
    [ DOI:10.1126/science.1231077 ]
  49. Sung, J.; Chen, B.-C.; Lim, S.-H., Fast Three-Dimensional Chemical Iimaging by Interferometric Multiplex Coherent Anti-Stokes Raman Scattering Microscopy. Journal of Raman Spectroscopy, 42, 130-136 (2011).
    [ DOI:10.1002/jrs.2647 ]
  50. Chen, B.-C.; Sung, J.; Wu, X.; Lim, S.-H., Chemical Imaging and Microspectroscopy with Spectral Focusing Coherent Anti-Stokes Raman Scattering (CARS). Journal of Biomedical Optics, 16, 021112 (2011).
    [ DOI:10.1117/1.3533315 ]
  51. Chen, B.-C.; Sung, J.; Lim, S.-H., Chemical Imaging with Frequency Modulation Coherent Anti-Stokes Raman Scattering microscopy at the vibrational Fingerprint Region. Journal of Physical Chemistry B, 114, 16871-16880 (2010).
    [ DOI:10.1021/jp104553s ]
  52. Chen, B.-C.; Lim, S.-H., Three-Dimensional Imaging of Direct field Orientations in Liquid Crystals by Polarized Four-Wave Mixing Microscopy. Applied Physics Letters, 94, 171911/1-171911/3 (2009).
    [ DOI:10.1063/1.3127535 ]
  53. Sung, J.; Chen, B.-C.; Lim, S.-H., Single-beam Homodyne SPIDER for Multiphoton Microscopy. Optics Letters, 33, 1404-1406 (2008). Selected for Virtual Journal of Ultrafast Science
    [ DOI:10.1364/OL.33.001404 ]
  54. Chen, B.-C.; Lim, S.-H., Optimal Laser Pulse Shaping for Interferometric Multiplex Coherent Anti-Stokes Raman Scattering Microscopy. Journal of Physical Chemistry B, 112, 3653-3661 (2008). Selected for the cover article
    [ DOI:10.1021/jp710743k ]
  55. Chen, B.-C.; Lim, S.-H., Characterization of a Broadband for Phased controlled Multiphoton Microscopy by Single Beam SPIDER. Optics Letter, 32, 2411-2413 (2007). Selected for Virtual Journal of Ultrafast Science.
    [ DOI:10.1364/OL.32.002411 ]
  56. Yeh, Y.-Q.; Chen, B.-C.; Lin, H.-P.; Tang, C.-Y., Synthesis of Hollow silica Spheres with Mesostructured Shell Using Cational-Anionic-Neutral Block Copolymer Ternary Surfactants. Langmuir, 22, 6-9 (2006).
    [ DOI:10.1021/la052129y ]
  57. Chen, B.-C.; Chao, M.-C.; Lin, H.-P; Mou, C.-Y., Faceted single Crystals of Mesoporous Silica SBA-16 from a Ternary Surfactant System : surface Roughening Model, Microporous and Mesoporous Material, 81, 241-249 (2005).
    [ DOI:10.1016/j.micromeso.2005.02.010 ]
  58. Chen, B.-C.; Lin, H.-P.; Chao, M.-C.; Mou, C.-Y.; Tang, C.-Y., Mesoporous silica Platelets with Perpendicular Nanochannel via a Ternary-Surfactant Template. Advanced Materials, 16, 1657-1661 (2004).
    [ DOI:10.1002/adma.200306327 ]