Ji-Yen Cheng 鄭郅言
Research Fellow and Deputy Director

Ways to contact me:

Fax: 02-2787-3122
Address: Research Center for Applied Sciences, Academia Sinica
128 Sec. 2, Academia Rd., Nankang, Taipei 11529, Taiwan, ROT
Adjunct Positions:
Dept. Mech. Mechatronic Eng., National Taiwan Ocean Univ. Taiwan, ROT
Inst. of Biophotonics, National Yang-Ming Univ. Taiwan, ROT



Research Fields:

  • Cell-based micro analysis:cell migration in weak DC EF, cell-cell interaction co-culture chip, cellular chemotaxis, electrotaxis and metastasis, affinity binding and separation.
  • Rapid prototyping of microfluidic biochip and their biomedical applications.
  • Microarray technologies: flexible in-situ array synthesis, rapid hybridization, mRNA labeling chip, and portable DNA amplification chip.
  • Laser micromachining – mechanism and applications.

Recent Publications:

  1. For up to date publications, go to : Publon:
  2. Chang, H.F. et. al. (2019) "Doxycycline inhibits electric field-induced migration of non-small cell lung cancer (NSCLC) cells", Sci. Reports, Nature Publishing Group, 9(8094)
    [ DOI:/10.1038/s41598-019-44505-8 ]
  3. Lu, M.-Y. and J.-Y. Cheng (2019). Optical Approaches to Visualization of Cellular Activity. Handbook of Cell Biosensors. Cham, Springer International Publishing. 9: 1-15.
    [ DOI:/10.1007/978-3-319-47405-2_127-1 ]
  4. Hou, H.-S., et al. (2019). "Simultaneous assessment of cell morphology and adhesion using aluminum nanoslit-based plasmonic biosensing chips."Sci. Reports, Nature Publishing Group 9(1): 7204.
    [ DOI:/10.1038/s41598-019-43442-w ]
  5. Zimmer, K., et al. (2018). "Towards fast nanopattern fabrication by local laser annealing of block copolymer (BCP) films." Applied surface science 470: 639-644. Applied Surface Science, 470 (2018) 639-644.
    [ DOI:/10.1016/j.apsusc.2018.11.105 ]
  6. Yeung, W. K., et al. (2018). "Multiplex detection of urinary miRNA biomarkers by transmission surface plasmon resonance." The Analyst 143(19): 4715-4722. Analyst (2018), 143, 4715-4722
    [ DOI:/10.1039/C8AN01127C ]
  7. Kao, W.-C., et al. (2018). "Microbial biosensing of ciprofloxacin residues in food by a portable lens-free CCD-based analyzer Wei-Chen Kao, Shimshon Belkin & Ji- Yen Cheng." 1-9. Anal Bioanal Chem
    [ DOI:/10.1007/s00216-017-0792-x ]
  8. Chen, W.-T., et al. (2018). "Alteration of mesenchymal stem cells polarity by laminar shear stimulation promoting β-catenin nuclear localization." Biomaterials 190-191: 1-10.
    [ DOI:/10.1016/j.biomaterials.2018.10.026 ]
  9. Huang, C.-H., et al. (2017). "Use microfluidic chips to study the effects of ultraviolet lights on human fibroblasts." Microfluidics and Nanofluidics 21(4): 7.
    [ DOI:/10.1034/j.1600-0781.2001.170407.x ]
  10. Cheng, J. Y., Kao, W. C., & Mousavi, M. Z. (2016). High-Quality Surface Micromachining on Polymer Using Visible-LIBWE. Journal of Laser Micro/ Nanoengineering.….
    [ DOI:/10.2961/jlmn.2016.01.0022 ]
  11. Li, Y.-C., Lin, M.-W., Yen, M.-H., Fan, M.-Y., Wu, J.-T., Young, T.-H., et al. (2015). Programmable laser-assisted surface microfabrication on a polyvinyl alcohol-coated glass chip with self-changing cell adhesivity for heterotypic cell patterning. ACS Applied Materials & Interfaces
    [ DOI:10.1021/acsami.5b05978 ]
  12. Lei, K. F., Wang, Y.-H., Chen, H.-Y., Sun, J.-H., & Cheng, J.-Y. (2015). Electrokinetic acceleration of DNA hybridization in microsystems. Talanta, 138(C), 149–154.
    [ DOI:10.1016/j.talanta.2015.02.024 ]
  13. Tsai, H.-F., Tsai, Y.-C., Yagur-Kroll, S., Palevsky, N., Belkin, S., & Cheng, J.-Y. (2015). Water pollutant monitoring by a whole cell array through lens-free detection on CCD. Lab on a Chip, 15(6), 1472–1480.
    [ DOI:/10.1039/c4lc01189a ]
  14. Mousavi, M., Chen, H.-Y., Hou, H.-S., Chang, C.-Y.-Y., Roffler, S., Wei, P.-K., & Cheng, J.-Y. (2015). Label-Free Detection of Rare Cell in Human Blood Using Gold Nano Slit Surface Plasmon Resonance. Biosensors, 5(1), 98–117.
    [ DOI:/10.3390/bios5010098 ]
  15. Mousavi, M. Z., Chen, H.-Y., Lee, K.-L., Lin, H., Chen, H.-H., Lin, Y.-F., et al. (2015). Urinary micro-RNA biomarker detection using capped gold nanoslit SPR in a microfluidic chip. The Analyst, 140(12), 4097–4104.
    [ DOI:/10.1039/C5AN00145E ]
  16. Hou, H.-S., Chang, H.-F., & Cheng, J.-Y. (2015). Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip. Journal of Visualized Experiments, (106).
    [ DOI:/10.3791/53340 ]
  17. Hu, J.-B., Chen, T.-R., Chang, C.-H., Cheng, J.-Y., Chen, Y.-C., & Urban, P. L. (2015). A compact 3D-printed interface for coupling open digital microchips with Venturi easy ambient sonic-spray ionization mass spectrometry. The Analyst, 140(5), 1495–1501.
    [ DOI:/10.1039/C4AN02220C ]
  18. Hou, H.-S., Tsai, H.-F., Chiu, H.-T., & Cheng, J.-Y. (2014). Simultaneous chemical and electrical stimulation on lung cancer cells using a multichannel-dual-electric-field chip. Biomicrofluidics, 8(5), 052007.
    [ DOI:/10.1063/1.4896296.2 ]
  19. Kao, Y.-C., Hsieh, M.-H., Liu, C.-C., Pan, H.-J., Liao, W.-Y., Cheng, J.-Y., et al. (2014). Modulating chemotaxis of lung cancer cells by using electric fields in a microfluidic device. Biomicrofluidics, 8(2), 024107.
    [ DOI:/10.1063/1.4870401 ]
  20. Pan, H.-J., Wang, R.-L., Xiao, J.-L., Chang, Y.-J., Cheng, J.-Y., Chen, Y.-R., & Lee, C.-H. (2014). Using optical profilometry to characterize cell membrane roughness influenced by amyloid-beta 42 aggregates and electric fields. Journal of Biomedical Optics, 19(1), 011009. http://doi.org/10.1117/1.JBO.19.1.011009
  21. M.Z. Mousavi, H.-Y. Chen, S.-H. Wu, S.-W. Peng, K.-L. Lee, P.-K. Wei, et al., Magnetic nanoparticle-enhanced SPR on gold nanoslits for ultra-sensitive, label-free detection of nucleic acid biomarkers, Analyst. 138 (2013) 2740.
  22. H.-F. Tsai, C.-W. Huang, H.-F. Chang, J.J.W. Chen, C.-H. Lee, J.-Y. Cheng, Evaluation of EGFR and RTK Signaling in the Electrotaxis of Lung Adenocarcinoma Cells under Direct-Current Electric Field Stimulation, PLoS ONE. 8 (2013) e73418.
  23. Ji-Yen Cheng, Mansoureh Z Mousavi, Chun-Ying Wu, Hsieh-Fu Tsai, Blue Light Plasma Emission During LIBWE Using 532 nm Q-switched Nanosecond Laser, JLMN-Journal of Laser Micro/Nanoengineering. 7 (2012) 87–92.
  24. Y.-S. Sun, S.-W. Peng, J.-Y. Cheng, In vitro electrical-stimulated wound-healing chip for studying electric field-assisted wound-healing process, Biomicrofluidics. 6 (2012) 034117–034112.
  25. Sun, Y.-S., S.-W. Peng, K.-H. Lin and J.-Y. Cheng* (2012). "Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds." Biomicrofluidics 6(1): 014102-14.
  26. Wang, C.-C., Y.-C. Ka, P.-Y. Chi, C.-W. Huang, J.-Y. Lin, C.-F. Chou, J.-Y. Cheng* and C.-H. Lee* (2011). "Asymmetric cancer-cell filopodium growth induced by electric-fields in a microfluidic culture chip." Lab on a Chip 11: 695–699.
  27. Huang, C.-W., H.-Y. Chen, M.-H. Yen, J. J. W. Chen, T.-H. Young and J.-Y. Cheng* (2011). "Gene Expression of Human Lung Cancer Cell Line CL1-5 in Response to a Direct Current Electric Field." PLoS ONE 6(10): e25928.
  28. Hsiung, L.-C., C.-L. Chiang, C.-H. Wang, Y.-H. Huang, C.-T. Kuo, J.-Y. Cheng, C.-H. Lin, V. Wu, H.-Y. Chou, D.-S. Jong, H. Lee and A. M. Wo* (2011). "Dielectrophoresis-based cellular microarray chip for anticancer drug screening in perfusion microenvironments." Lab on a Chip 11(14): 2333-2342.
  29. Cheng*, J.-Y., M. Z. Mousavi, C.-Y. Wu and H.-F. Tsai (2011). "Blue light emission from glass/liquid interface for real-time monitoring of laser-induced etching process." Journal of Micromechanics and Microengineering 21: 075019
  30. Hsiao, T. H., C. H. Lin, T. T. Lee, J. Y. Cheng, P. K. Wei, E. Y. Chuang and K. Peck* (2010). "Verifying expressed transcript variants by detecting and assembling stretches of consecutive exons." Nucleic Acids Research 38(20): 13
  31. Cheng, J.-Y., Tsai, C.-G., & Lee, S.-C. (2010). Bamboos as the material for saxophone reed. Presented at the International Congress on Acoustics, ICA 2010, Sydney Australia.
  32. Yen, M.-H., C.-W. Huang, W.-C. Hsu, T.-H. Young, K. Zimmer and J.-Y. Cheng* (2010). "Crack-free micromachining on glass substrates by visible LIBWE using liquid metallic absorbers." Applied Surface Science 257(1): 87-92.
  33. Microfluidic ARray Synthesizer (MArS) for Rapid Preparation and Hybridization of
    Custom DNA Microarray Ji-Yen Cheng* and Huai-Yi Chen, Biotechnology and Bioengineering, 2009, V104, p400
    [ DOI:10.1002/bit.22383 ]
  34. T.-H. Hsu, M.-H. Yen, W.-Y. Liao, J.-Y. Cheng* and C.-H. Lee*, "Label-free quantification of asymmetric cancer-cell filopodium activities in a multi-gradient chip ", Lab on a Chip, 9, 884-890.(2009)
    [ DOI:10.1039/b814405b ]
  35. C.-W. Huang, J.-Y. Cheng*, M.-H. Yen and T.-H. Younga, "Electrotaxis of lung cancer cells in a multiple-electric-field chip", Biosensors and Bioelectronics, 24, 3510-3516.(2009)
    [ DOI:10.1016/j.bios.2009.05.001 ]
  36. L.-C. Hsiung, C.-H. Yang, C.-L. Chiu, C.-L. Chen, YuehWang, H. Lee, J.-Y. Cheng, M.-C. Ho and A. M.Wo*, "A planar interdigitated ring electrode array via dielectrophoresis for uniform patterning of cells", Biosensors and Bioelectronics, 24, 875-881.(2008)
    [ DOI:10.1016/j.bios.2008.07.027 ]
  37. J.-Y. Cheng*, M.-H. Yen, C.-T. Kuo and T.-H. Young, "A transparent cell culture microchamber with variablly controlled concentration gradient generator and flow field rectifier", Biomicrofluidics, 2, 024105.(2008)
    [ DOI:10.1063/1.2952290 ]
  38. Cheng, J.-Y., Yen, M.-H., Hsu, W.-C., Jhang, J.-H. and Young, T.-H. (2007) ITO patterning by a low power Q-switched green laser and its use in the fabrication of a transparent flow meter. J. Micromech. Microeng., 17, 2316-2323
    [ DOI:10.1088/0960-1317/17/11/019 ]
  39. Yen, M.-H., Cheng, J.-Y., Wei, C.-W., Chuang, Y.-C. and Young, T.-H. (2006) Rapid cell-patterning and microfluidic chip fabrication by crack-free CO2 laser ablation on glass. Journal of Micromechanics and Microengineering, 16, 1143-1153.
  40. Wei, C.-W., Cheng, J.-Y. and Young, T.-H. (2006) Elucidating in vitro Cell-Cell Interaction Using a Microfluidic Coculture System. Biomedical Microdevices, 8, 65-71.
  41. Cheng, J.-Y., Yen, M.-H. and Young, T.-H. (2006) Crack-free micromachining on glass using an economic green laser. Journal of Micromechanics and Microengineering, 16, 2420-2424
  42. Wei, C.-W., Young, T.-H. and Cheng, J.-Y. (2005) Electroosmotic mixing induced by non-uniform zeta potential and application for DNA microarray in microfluidic channel (EI). Biomedical Engineering Applications, Basis & Communications, 17, 281-283.
  43. Wei, C.-W., Cheng, J.-Y., Huang, C.-T., Yen, M.-H. and Young, T.-H. (2005) Using a microfluidic device for 1 microliter DNA microarray hybridization in 500 seconds. Nucleic Acids Research Methods, 33, e78. Times cited: 8

    [ DOI:10.1093/nar/gni078 ]
  44. Kang, C.-C., Chang, C.-C., Cheng, J.-Y. and Chang, T.-C. (2005) Simple method in diagnosing cancer cells by a novel fluorescence probe BMVC. J. Chin. Chem. Soc., 52, 1069-1072.
  45. Cheng, J.-Y., Yen, M.-H., Wei, C.-W., Chuang, Y.-C. and Young, T.-H. (2005) Crack-free direct-writing on glass using a low-power UV laser in the manufacture of a microfluidic chip. Journal of Micromechanics and Microengineering, 15, 1147-1156. Times cited: 1, including 1 Research Highlight in Lab-on-Chip, Oct, 2005.
  46. Cheng, J.-Y., Hsieh, C.-J. and Chuang, Y.-C. (2005) Performing microchannel temperature cycling reactions using reciprocating reagent shuttling along radial temperature gradient. The Analyst, 130, 931-940. Times cited: 2, including 1 Research Highlight in Lab-on-Chip, Sep, 2005.
  47. Chou, R.-H., Lin, K.-C., Lin, S.-C., Cheng, J.-Y., Wu, C.-W. and Chang, W.-S.W. (2004) Cost-effective trapezoidal modified Boyden chamber with comparable accuracy to a commercial. Biotechniques, 37, 724-726.
  48. Cheng, J.-Y., Wei, C.-W., Hsu, K.-H. and Young, T.-H. (2004) Direct-write Laser Micromachining and Universal Surface Modification of PMMA for Device Development. Sensors and Actuators B:Chemistry, 99, 186-196. Times cited: 14. Selected in the Prominent Research Accomplishment of Academia Sinica, 2005 (94年中央研究院重要研究成果)
  49. Cheng, J.-Y. and Hsiung, L.-C. (2004) Electrowetting (EW)-Based Valve Combined with Hydrophilic Teflon Microfluidic Guidance in Controlling Continuous Fluid Flow. Biomedical Microdevices, 6, 341-347. Times cited: 1
  50. Cheng, J.-Y., Chen, H.-H., Kao, Y.-S., Kao, W.-C. and Peck, K. (2002) High throughput parallel synthesis of oligonucleotides with 1536 channel synthesizer. Nucleic Acids Research Methods, 30, e93.



    Patent Granted: Europe, United Kingdom, France, 3350310, 2019, May 22
    Provisional, US, Application number: 62/220,841, Application date 2018, Sep, 15
  2. "雷射無碎裂微加工 Crack-free Glass Micromachining Using Laser", Cheng, J.-Y., 鄭郅言, Yen, M.-H. and 顏孟華. (2007) 台灣 patent no. I271252.
  3. "生化反應晶片及其製法 Temperature Cycling Reaction Chip for Biological Reaction" Cheng, J.-Y., 鄭郅言, Hsieh, H.-J., 謝茜如, Chuang, Y.-C. and 莊永全. (2007) 台灣 patent no. I290954.
  4. “流體驅動裝置及其製法”, “Apparatus and fabrication of microfluidic driving device”, Patent Granted, Taiwan Patent No. 258456, Jul, 2006.
  5. "多管道寡核苷酸合成方法及裝置 Multi-channel Oligonucleotide Synthesis - Method and Device", Peck, K., 白果能, Cheng, J.-Y. and 鄭郅言. (2005) 台灣 patent no. I232867.
  6. “Apparatus and Methods for Chemical Synthesis”, Konan Peck, Ji-Yen Cheng, 2002, Patent Granted, US Pat. No.6,867,050.