Ji-Yen Cheng 鄭郅言
Research Fellow and Deputy Director

Ways to contact me：

E-mail:jychenggate.sinica.edu.tw
Phone:886-2-2787-3136
Fax: 02-2787-3122
Address: Research Center for Applied Sciences, Academia Sinica
128 Sec. 2, Academia Rd., Nankang, Taipei 11529, Taiwan, ROT
中央研究院應用科學研究中心
台北市11529南港區研究院路二段128號
Adjunct Positions:
Dept. Mech. Mechatronic Eng., National Taiwan Ocean Univ. Taiwan, ROT
Inst. of Biophotonics, National Yang-Ming Univ. Taiwan, ROT

Education：

Experience：

Research Fields：

  • Cell-based micro analysis：cell migration in weak DC EF, cell-cell interaction co-culture chip, cellular chemotaxis, electrotaxis and metastasis, affinity binding and separation.

  • Rapid prototyping of microfluidic biochip and their biomedical applications.

  • Microarray technologies： flexible in-situ array synthesis, rapid hybridization, mRNA labeling chip, and portable DNA amplification chip.

  • Laser micromachining – mechanism and applications.

Recent Publications：

  1. For up to date publications, go to ： Publon：
    https：//www.webofscience.com/wos/author/record/D-7272-2011

  2. Chang, H.F. et. al. (2019) "Doxycycline inhibits electric field-induced migration of non-small cell lung cancer (NSCLC) cells", Sci. Reports, Nature Publishing Group, 9(8094)
    [ DOI:/10.1038/s41598-019-44505-8 ]

  3. Lu, M.-Y. and J.-Y. Cheng (2019). Optical Approaches to Visualization of Cellular Activity. Handbook of Cell Biosensors. Cham, Springer International Publishing. 9： 1-15.
    [ DOI:/10.1007/978-3-319-47405-2_127-1 ]

  4. Hou, H.-S., et al. (2019). "Simultaneous assessment of cell morphology and adhesion using aluminum nanoslit-based plasmonic biosensing chips."Sci. Reports, Nature Publishing Group 9(1)： 7204.
    [ DOI:/10.1038/s41598-019-43442-w ]

  5. Zimmer, K., et al. (2018). "Towards fast nanopattern fabrication by local laser annealing of block copolymer (BCP) films." Applied surface science 470： 639-644. Applied Surface Science, 470 (2018) 639-644.
    [ DOI:/10.1016/j.apsusc.2018.11.105 ]

  6. Yeung, W. K., et al. (2018). "Multiplex detection of urinary miRNA biomarkers by transmission surface plasmon resonance." The Analyst 143(19)： 4715-4722. Analyst (2018), 143, 4715-4722
    [ DOI:/10.1039/C8AN01127C ]

  7. Kao, W.-C., et al. (2018). "Microbial biosensing of ciprofloxacin residues in food by a portable lens-free CCD-based analyzer Wei-Chen Kao, Shimshon Belkin & Ji- Yen Cheng." 1-9. Anal Bioanal Chem
    [ DOI:/10.1007/s00216-017-0792-x ]

  8. Chen, W.-T., et al. (2018). "Alteration of mesenchymal stem cells polarity by laminar shear stimulation promoting β-catenin nuclear localization." Biomaterials 190-191： 1-10.
    [ DOI:/10.1016/j.biomaterials.2018.10.026 ]

  9. Huang, C.-H., et al. (2017). "Use microfluidic chips to study the effects of ultraviolet lights on human fibroblasts." Microfluidics and Nanofluidics 21(4)： 7.
    [ DOI:/10.1034/j.1600-0781.2001.170407.x ]

  10. Cheng, J. Y., Kao, W. C., & Mousavi, M. Z. (2016). High-Quality Surface Micromachining on Polymer Using Visible-LIBWE. Journal of Laser Micro/ Nanoengineering.….
    [ DOI:/10.2961/jlmn.2016.01.0022 ]

  11. Li, Y.-C., Lin, M.-W., Yen, M.-H., Fan, M.-Y., Wu, J.-T., Young, T.-H., et al. (2015). Programmable laser-assisted surface microfabrication on a polyvinyl alcohol-coated glass chip with self-changing cell adhesivity for heterotypic cell patterning. ACS Applied Materials & Interfaces
    [ DOI:10.1021/acsami.5b05978 ]

  12. Lei, K. F., Wang, Y.-H., Chen, H.-Y., Sun, J.-H., & Cheng, J.-Y. (2015). Electrokinetic acceleration of DNA hybridization in microsystems. Talanta, 138(C), 149–154.
    [ DOI:10.1016/j.talanta.2015.02.024 ]

  13. Tsai, H.-F., Tsai, Y.-C., Yagur-Kroll, S., Palevsky, N., Belkin, S., & Cheng, J.-Y. (2015). Water pollutant monitoring by a whole cell array through lens-free detection on CCD. Lab on a Chip, 15(6), 1472–1480.
    [ DOI:/10.1039/c4lc01189a ]

  14. Mousavi, M., Chen, H.-Y., Hou, H.-S., Chang, C.-Y.-Y., Roffler, S., Wei, P.-K., & Cheng, J.-Y. (2015). Label-Free Detection of Rare Cell in Human Blood Using Gold Nano Slit Surface Plasmon Resonance. Biosensors, 5(1), 98–117.
    [ DOI:/10.3390/bios5010098 ]

  15. Mousavi, M. Z., Chen, H.-Y., Lee, K.-L., Lin, H., Chen, H.-H., Lin, Y.-F., et al. (2015). Urinary micro-RNA biomarker detection using capped gold nanoslit SPR in a microfluidic chip. The Analyst, 140(12), 4097–4104.
    [ DOI:/10.1039/C5AN00145E ]

  16. Hou, H.-S., Chang, H.-F., & Cheng, J.-Y. (2015). Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip. Journal of Visualized Experiments, (106).
    [ DOI:/10.3791/53340 ]

  17. Hu, J.-B., Chen, T.-R., Chang, C.-H., Cheng, J.-Y., Chen, Y.-C., & Urban, P. L. (2015). A compact 3D-printed interface for coupling open digital microchips with Venturi easy ambient sonic-spray ionization mass spectrometry. The Analyst, 140(5), 1495–1501.
    [ DOI:/10.1039/C4AN02220C ]

  18. Hou, H.-S., Tsai, H.-F., Chiu, H.-T., & Cheng, J.-Y. (2014). Simultaneous chemical and electrical stimulation on lung cancer cells using a multichannel-dual-electric-field chip. Biomicrofluidics, 8(5), 052007.
    [ DOI:/10.1063/1.4896296.2 ]

  19. Kao, Y.-C., Hsieh, M.-H., Liu, C.-C., Pan, H.-J., Liao, W.-Y., Cheng, J.-Y., et al. (2014). Modulating chemotaxis of lung cancer cells by using electric fields in a microfluidic device. Biomicrofluidics, 8(2), 024107.
    [ DOI:/10.1063/1.4870401 ]

  20. Pan, H.-J., Wang, R.-L., Xiao, J.-L., Chang, Y.-J., Cheng, J.-Y., Chen, Y.-R., & Lee, C.-H. (2014). Using optical profilometry to characterize cell membrane roughness influenced by amyloid-beta 42 aggregates and electric fields. Journal of Biomedical Optics, 19(1), 011009. http：//doi.org/10.1117/1.JBO.19.1.011009

  21. M.Z. Mousavi, H.-Y. Chen, S.-H. Wu, S.-W. Peng, K.-L. Lee, P.-K. Wei, et al., Magnetic nanoparticle-enhanced SPR on gold nanoslits for ultra-sensitive, label-free detection of nucleic acid biomarkers, Analyst. 138 (2013) 2740.

  22. H.-F. Tsai, C.-W. Huang, H.-F. Chang, J.J.W. Chen, C.-H. Lee, J.-Y. Cheng, Evaluation of EGFR and RTK Signaling in the Electrotaxis of Lung Adenocarcinoma Cells under Direct-Current Electric Field Stimulation, PLoS ONE. 8 (2013) e73418.

  23. Ji-Yen Cheng, Mansoureh Z Mousavi, Chun-Ying Wu, Hsieh-Fu Tsai, Blue Light Plasma Emission During LIBWE Using 532 nm Q-switched Nanosecond Laser, JLMN-Journal of Laser Micro/Nanoengineering. 7 (2012) 87–92.

  24. Y.-S. Sun, S.-W. Peng, J.-Y. Cheng, In vitro electrical-stimulated wound-healing chip for studying electric field-assisted wound-healing process, Biomicrofluidics. 6 (2012) 034117–034112.

  25. Sun, Y.-S., S.-W. Peng, K.-H. Lin and J.-Y. Cheng* (2012). "Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds." Biomicrofluidics 6(1)： 014102-14.

  26. Wang, C.-C., Y.-C. Ka, P.-Y. Chi, C.-W. Huang, J.-Y. Lin, C.-F. Chou, J.-Y. Cheng* and C.-H. Lee* (2011). "Asymmetric cancer-cell filopodium growth induced by electric-fields in a microfluidic culture chip." Lab on a Chip 11： 695–699.

  27. Huang, C.-W., H.-Y. Chen, M.-H. Yen, J. J. W. Chen, T.-H. Young and J.-Y. Cheng* (2011). "Gene Expression of Human Lung Cancer Cell Line CL1-5 in Response to a Direct Current Electric Field." PLoS ONE 6(10)： e25928.

  28. Hsiung, L.-C., C.-L. Chiang, C.-H. Wang, Y.-H. Huang, C.-T. Kuo, J.-Y. Cheng, C.-H. Lin, V. Wu, H.-Y. Chou, D.-S. Jong, H. Lee and A. M. Wo* (2011). "Dielectrophoresis-based cellular microarray chip for anticancer drug screening in perfusion microenvironments." Lab on a Chip 11(14)： 2333-2342.

  29. Cheng*, J.-Y., M. Z. Mousavi, C.-Y. Wu and H.-F. Tsai (2011). "Blue light emission from glass/liquid interface for real-time monitoring of laser-induced etching process." Journal of Micromechanics and Microengineering 21： 075019

  30. Hsiao, T. H., C. H. Lin, T. T. Lee, J. Y. Cheng, P. K. Wei, E. Y. Chuang and K. Peck* (2010). "Verifying expressed transcript variants by detecting and assembling stretches of consecutive exons." Nucleic Acids Research 38(20)： 13

  31. Cheng, J.-Y., Tsai, C.-G., & Lee, S.-C. (2010). Bamboos as the material for saxophone reed. Presented at the International Congress on Acoustics, ICA 2010, Sydney Australia.

  32. Yen, M.-H., C.-W. Huang, W.-C. Hsu, T.-H. Young, K. Zimmer and J.-Y. Cheng* (2010). "Crack-free micromachining on glass substrates by visible LIBWE using liquid metallic absorbers." Applied Surface Science 257(1)： 87-92.

  33. Microfluidic ARray Synthesizer (MArS) for Rapid Preparation and Hybridization of
    Custom DNA Microarray Ji-Yen Cheng* and Huai-Yi Chen, Biotechnology and Bioengineering, 2009, V104, p400
    [ DOI:10.1002/bit.22383 ]

  34. T.-H. Hsu, M.-H. Yen, W.-Y. Liao, J.-Y. Cheng* and C.-H. Lee*, "Label-free quantification of asymmetric cancer-cell filopodium activities in a multi-gradient chip ", Lab on a Chip, 9, 884-890.(2009)
    [ DOI:10.1039/b814405b ]

  35. C.-W. Huang, J.-Y. Cheng*, M.-H. Yen and T.-H. Younga, "Electrotaxis of lung cancer cells in a multiple-electric-field chip", Biosensors and Bioelectronics, 24, 3510-3516.(2009)
    [ DOI:10.1016/j.bios.2009.05.001 ]

  36. L.-C. Hsiung, C.-H. Yang, C.-L. Chiu, C.-L. Chen, YuehWang, H. Lee, J.-Y. Cheng, M.-C. Ho and A. M.Wo*, "A planar interdigitated ring electrode array via dielectrophoresis for uniform patterning of cells", Biosensors and Bioelectronics, 24, 875-881.(2008)
    [ DOI:10.1016/j.bios.2008.07.027 ]

  37. J.-Y. Cheng*, M.-H. Yen, C.-T. Kuo and T.-H. Young, "A transparent cell culture microchamber with variablly controlled concentration gradient generator and flow field rectifier", Biomicrofluidics, 2, 024105.(2008)
    [ DOI:10.1063/1.2952290 ]

  38. Cheng, J.-Y., Yen, M.-H., Hsu, W.-C., Jhang, J.-H. and Young, T.-H. (2007) ITO patterning by a low power Q-switched green laser and its use in the fabrication of a transparent flow meter. J. Micromech. Microeng., 17, 2316-2323
    [ DOI:10.1088/0960-1317/17/11/019 ]

  39. Yen, M.-H., Cheng, J.-Y., Wei, C.-W., Chuang, Y.-C. and Young, T.-H. (2006) Rapid cell-patterning and microfluidic chip fabrication by crack-free CO2 laser ablation on glass. Journal of Micromechanics and Microengineering, 16, 1143-1153.

  40. Wei, C.-W., Cheng, J.-Y. and Young, T.-H. (2006) Elucidating in vitro Cell-Cell Interaction Using a Microfluidic Coculture System. Biomedical Microdevices, 8, 65-71.

  41. Cheng, J.-Y., Yen, M.-H. and Young, T.-H. (2006) Crack-free micromachining on glass using an economic green laser. Journal of Micromechanics and Microengineering, 16, 2420-2424

  42. Wei, C.-W., Young, T.-H. and Cheng, J.-Y. (2005) Electroosmotic mixing induced by non-uniform zeta potential and application for DNA microarray in microfluidic channel (EI). Biomedical Engineering Applications, Basis & Communications, 17, 281-283.

  43. Wei, C.-W., Cheng, J.-Y., Huang, C.-T., Yen, M.-H. and Young, T.-H. (2005) Using a microfluidic device for 1 microliter DNA microarray hybridization in 500 seconds. Nucleic Acids Research Methods, 33, e78. Times cited： 8

    [ DOI:10.1093/nar/gni078 ]

  44. Kang, C.-C., Chang, C.-C., Cheng, J.-Y. and Chang, T.-C. (2005) Simple method in diagnosing cancer cells by a novel fluorescence probe BMVC. J. Chin. Chem. Soc., 52, 1069-1072.

  45. Cheng, J.-Y., Yen, M.-H., Wei, C.-W., Chuang, Y.-C. and Young, T.-H. (2005) Crack-free direct-writing on glass using a low-power UV laser in the manufacture of a microfluidic chip. Journal of Micromechanics and Microengineering, 15, 1147-1156. Times cited： 1, including 1 Research Highlight in Lab-on-Chip, Oct, 2005.

  46. Cheng, J.-Y., Hsieh, C.-J. and Chuang, Y.-C. (2005) Performing microchannel temperature cycling reactions using reciprocating reagent shuttling along radial temperature gradient. The Analyst, 130, 931-940. Times cited： 2, including 1 Research Highlight in Lab-on-Chip, Sep, 2005.

  47. Chou, R.-H., Lin, K.-C., Lin, S.-C., Cheng, J.-Y., Wu, C.-W. and Chang, W.-S.W. (2004) Cost-effective trapezoidal modified Boyden chamber with comparable accuracy to a commercial. Biotechniques, 37, 724-726.

  48. Cheng, J.-Y., Wei, C.-W., Hsu, K.-H. and Young, T.-H. (2004) Direct-write Laser Micromachining and Universal Surface Modification of PMMA for Device Development. Sensors and Actuators B：Chemistry, 99, 186-196. Times cited： 14. Selected in the Prominent Research Accomplishment of Academia Sinica, 2005 (94年中央研究院重要研究成果)

  49. Cheng, J.-Y. and Hsiung, L.-C. (2004) Electrowetting (EW)-Based Valve Combined with Hydrophilic Teflon Microfluidic Guidance in Controlling Continuous Fluid Flow. Biomedical Microdevices, 6, 341-347. Times cited： 1

  50. Cheng, J.-Y., Chen, H.-H., Kao, Y.-S., Kao, W.-C. and Peck, K. (2002) High throughput parallel synthesis of oligonucleotides with 1536 channel synthesizer. Nucleic Acids Research Methods, 30, e93.

Patents：

  1. 3D POLYMERIC INSERT TO APPLY UNIFORM ELECTRIC FIELD IN CIRCULAR CULTUREWARE 傳統細胞培養皿用之高效能均勻電場刺激裝置

    Patent Granted： Europe, United Kingdom, France, 3350310, 2019, May 22
    Provisional, US, Application number： 62/220,841, Application date 2018, Sep, 15

  2. "雷射無碎裂微加工 Crack-free Glass Micromachining Using Laser", Cheng, J.-Y., 鄭郅言, Yen, M.-H. and 顏孟華. (2007) 台灣 patent no. I271252.

  3. "生化反應晶片及其製法 Temperature Cycling Reaction Chip for Biological Reaction" Cheng, J.-Y., 鄭郅言, Hsieh, H.-J., 謝茜如, Chuang, Y.-C. and 莊永全. (2007) 台灣 patent no. I290954.

  4. “流體驅動裝置及其製法”, “Apparatus and fabrication of microfluidic driving device”, Patent Granted, Taiwan Patent No. 258456, Jul, 2006.

  5. "多管道寡核苷酸合成方法及裝置 Multi-channel Oligonucleotide Synthesis - Method and Device", Peck, K., 白果能, Cheng, J.-Y. and 鄭郅言. (2005) 台灣 patent no. I232867.

  6. “Apparatus and Methods for Chemical Synthesis”, Konan Peck, Ji-Yen Cheng, 2002, Patent Granted, US Pat. No.6,867,050.