Narrative CV

陳壁彰，2001年自台大化學系學士畢業，2003年台大化學所畢業，研究中孔洞材料的型態變化。於2011年，美國德州大學奧斯汀分校化學暨生化系博士畢業，從事非線性拉曼光學微顯鏡技術，在化學影像上的生物應用。2011至2014於美國霍華休斯醫學院(Howard Hughes Medical Institute)和2014年諾貝爾化學獎得主Eric Betzig 從事博士後研究，開發「晶格層光顯微鏡」，針對快速三維活體螢光掃描，此工作獲得AAAS Newcomb Cleveland Prize。於2014年加入中央研究院應用科學中心，擔任助研究員，2025年升為研究員迄今，主要進行開發三維大組織之高解析層光顯微鏡，利用單分子影像技術『透化層光定位顯微鏡』可以成功的看到記憶蛋白質分子在全果蠅腦組織中的空間分佈。及樣品膨脹技術『聚丙烯酸鉀膨脹層光奈米顯微術』，結合了樣品放大術及層光顯微鏡，讓光學顯微鏡在三維的大組織影像，可以有趨近電子顯微鏡的空間解析且擁有多色的螢光染色標記。未來期望可以將光學顯微術的解析度達到電子顯微鏡之解析度。此相關研究成果曾獲第十八屆有庠科技論文獎，中央研究院年輕學者研究成果獎，亦獲得中研院前瞻計畫，及科技部優秀年輕學者研究計畫補助。

Education

• 1997~2001, B.S., Department of Chemistry, National Taiwan University, Taipei, Taiwan
• 2001~2003, M.S., Department of Chemistry, National Taiwan University, Taipei, Taiwan
• 2006~2011, Ph. D., Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX, USA

Positions and Career

• 中央研究院應用科學研究中心副主任 (2026–Present)
• 中央研究院應用科學研究中心研究員 (2025–Present)
• 中央研究院應用科學研究中心副研究員 (2020–2025)
• 中央研究院應用科學研究中心助研究員 (2014–2020)
• 美國霍華休斯醫學院Janelia研究院博士後 (2011–2014)

Honors and Awards

• 2015 美國科學促進會(AAAS)紐科姆·克利夫蘭獎(Newcomb Cleveland Prize)
• 2020 第十八屆有庠科技論文獎-光電組
• 2021 中央研究院年輕學者研究成果獎

Selected Publications

• W.-C. Tang, Y.-T. Liu, C.-H. Yeh, Y.-L. Lin, Y.-C. Lin, T.-L. Hsu, L. Gao, P. Chen*, and B.-C. Chen*, "Optogenetic Manipulation of Cell Migration with High Spatiotemporal Resolution Using Lattice Lightsheet Microscopy" Communications Biology , 5, 879, DOI:10.1038/s42003- 022-03835-6 (2022)
• Fan, Y.-J.*; Hsieh, H.-Y.; Huang, Y.-R.; Tsao, C.; Lee, C.-M.; Tahara,H.; Wu,Y.-C.; Sheen, H.-J.*; Chen, B.-C.*, "Development of a water refractive index-matched microneedle integrated into a light sheet microscopy system for continuous embryonic cell imaging", Lab on a chip , 22, 584-591 DOI:10.1039/D1LC00827G (2022)
• Lin, M.-H., Chen, J.-C., Tian, X., Lee, C.-M., Yu,I-S., Lo, Y.-F., Uchida, S., Huang, C.-L., Chen, B.-C.*; Cheng, C.-J.*, "Impairment in renal medulla development underlies salt wasting in Clc-k2 channel deficiency", JCI Insight , 9:151039 ; DOI:10.1172/jci.insight (2021) selected as the cover image
• Fan, Y.-J.*, Hsieh, H.-Y., Tsai, S.-F., Wu, C.-H., Lee, C.-M., Liu, Y.-T., Lu, C.-H., Chang, S.-W.*, Chen, B.-C.*, "Microfluidic channel integrated with a lattice lightsheet microscopic system for continuous cell imaging" Lab on a chip , 21, 344 DOI: 10.1039/d0lc01009j (2021)
• Tsai, Y.-C.; Tang, W.-C.; Low, C. S. L.; Liu, Y.-T.; Wu, J.-S.; Lee, P.-Y.; Lin, Y.-L.; Kanchanawong, P.; Gao, L, and Chen, B.-C.* "Rapid High Resolution 3D Imaging of Expanded Biological Specimens with Lattice Lightsheet Microscopy", Methods , 174, 11-19 DOI:10.1016/j.ymeth.2019.04.006 (2020)
• Chu, L.-A., Lu, C.-H., Yang, S.-M., Liu, Y-T, Feng, K.-L., Tsai, Y.-C., Chang, W.-K., Wang, W.-C., Chang, S.-W., Chen, P.; Lee, T.-K., Hwu, Y.-K., Chiang, A.-S.*; and Chen, B.-C.*, "Rapid single-wavelength lightsheet localization microscopy for clarified tissue" Nature Communications, 10, 4762, DOI: 10.1038/s41467-019-12715-3 (2019)
• Lu, C.-H.; Tang, W.-C.; Liu, Y.-T.; Wu, F. C. M.; Chen, C. Y.; Tsai, Y. C.; Yang, S.-M; Kuo, C.-W.; Okada, Y.; Hwu, Y.-K.; Chang, S.-W.; Chen, P. * and Chen, B.-C.*, "Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging." Communications Biology, 2, 177 DOI:10.1038/s42003-019-0403-9 (2019)
• Gao, L. *; Tang,W.-C.; Tsai, Y.-C.; Chen, B.-C.* "Lattice light sheet microscopy using tiling lattice light sheets" Opt. Express, 27 1497- 1506 DOI:10.1364/OE.27.001497 (2019)

• Tian, X.; Lin, T.-Y., L; Lin, P.-T., Tsai; M.-J., Chen, H.; Chen, W.-J.; Lee, C.-M.; Tu, C.-H.; Hsu, J.-C.; Hsieh, T.-H.; Tung, Y.-C.; Wang, C.-K.; Lin, S.; Chu, L.-A.; Tseng, F.-G.; Hsueh, Y.-P., Lee, C.-H.; Chen, P.; Chen, B.-C.* "Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassium acrylate-based expansion microscopy", Nature Communications, 15, 10911 DOI:10.1038/s41467-024-55305-8 (2024)
• Wang, U.-T. T.; Tian, X.; Liou, Y.-H.; Lee, S.-P.; Hu H.-T.; Lu, C.-H.; Lin, P.-T.; Cheng, Y.-J.; Chen P. and Chen, B.-C.* "Protein and lipid expansion microscopy with trypsin and tyramide signal amplification for 3D imaging", Scientific Reports, 13, 21922 DOI:10.1038/s41598-023-48959-9 (2023)
• Tang, W.-C.; Liu, Y.-T.; Yeh, C.-H.; Lin, Y.-L.; Lin, Y.-C.; Hsu, T.-L.; Gao, L.; Chen*, P. and B.-C. Chen*, "Optogenetic Manipulation of Cell Migration with High Spatiotemporal Resolution Using Lattice Lightsheet Microscopy" Communications Biology, 5, 879, DOI:10.1038/s42003-022-03835-6 (2022)
• Chu, L.-A., Lu, C.-H., Yang, S.-M., Liu, Y-T, Feng, K.-L., Tsai, Y.-C., Chang, W.-K., Wang, W.-C., Chang, S.-W., Chen, P.; Lee, T.-K., Hwu, Y.-K., Chiang, A.-S. *; and Chen, B.-C.*, "Rapid single-wavelength lightsheet localization microscopy for clarified tissue" Nature Communications, 10, 4762, DOI: 10.1038/s41467-019-12715-3 (2019)
• Lu, C.-H.; Tang, W.-C.; Liu, Y.-T.; Wu, F. C. M.; Chen, C. Y.; Tsai, Y. C.; Yang, S.-M; Kuo, C.-W.; Okada, Y.; Hwu, Y.-K.; Chang, S.-W.; Chen, P. * and Chen, B.-C.*, “Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging.” Communications Biology, 2, 177 DOI:10.1038/s42003-019-0403-9 (2019)