Narrative CV

Bi-Chang Chen, an Research Fellow specializing in lightsheet microscopy. His research interests focus on superresolution lightsheet imaging in cleared and expanded tissues. He obtained Ph.D. degree in chemistry at the University of Texas at Austin and have contributed to multiple international research projects.

He developed lightsheet localization microscopy for clarified tissue and potassium acrylate expansion lightsheet microscopy, now applied for most of the model organisms, impacting neuroscience and developmental biology.

He has collaborated with Institute of Cellular and Organismic Biology, Institute of Molecular Biology and Biodiversity Research Center on expanded fly/mouse brain neuroscience and plant tissue clearing leading to the whole mouse brain imaging, coral-associated microbial aggregates in the cleared coral imaging and cleared imaging on fungus-cyanobacterium biosystem. As a mentor, he has guided 10 graduate students, fostering new talent in imaging techniques in biological applications and also serve as a reviewer for Cell, Science Advance, Nature Methods, Nature Communications, Scientific Reports, Biomedical Optical Express, Cell Report Method, SPIE journal, Optica, ACS photonics and contribute to Biophysical Society Annual Meetings.

His future research aims to deepen the increase of optical spatial resolution approaching the electron microscopy’s power and enhance interdisciplinary collaboration.

Education

• 1997~2001, B.S., Department of Chemistry, National Taiwan University, Taipei, Taiwan
• 2001~2003, M.S., Department of Chemistry, National Taiwan University, Taipei, Taiwan
• 2006~2011, Ph. D., Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX, USA

Positions and Career

• Deputy Director, Research Center for Applied Sciences Academia Sinica (2026–Present)
• Research Fellow, Research Center for Applied Sciences Academia Sinica (2025–Present)
• Associate Research Fellow, Research Center for Applied Sciences Academia Sinica (2020–2025)
• Assistant Research Fellow, Research Center for Applied Sciences Academia Sinica (2014–2020)
• Postdoctoral Associate, Howard Hughes Medical Institute/Janelia Research Campus, USA (2011–2014)

Honors and Awards

• The 2015 AAAS Newcomb Cleveland Prize
• 2020 18th Y. Z. Hsu Science Paper Award, Optoelectronics Science & Technology Category
• 2021 Academia Sinica Early-Career Investigator Research Achievement Award

Selected Publications

• W.-C. Tang, Y.-T. Liu, C.-H. Yeh, Y.-L. Lin, Y.-C. Lin, T.-L. Hsu, L. Gao, P. Chen*, and B.-C. Chen*, "Optogenetic Manipulation of Cell Migration with High Spatiotemporal Resolution Using Lattice Lightsheet Microscopy" Communications Biology , 5, 879, DOI:10.1038/s42003- 022-03835-6 (2022)
• Fan, Y.-J.*; Hsieh, H.-Y.; Huang, Y.-R.; Tsao, C.; Lee, C.-M.; Tahara,H.; Wu,Y.-C.; Sheen, H.-J.*; Chen, B.-C.*, "Development of a water refractive index-matched microneedle integrated into a light sheet microscopy system for continuous embryonic cell imaging", Lab on a chip , 22, 584-591 DOI:10.1039/D1LC00827G (2022)
• Lin, M.-H., Chen, J.-C., Tian, X., Lee, C.-M., Yu,I-S., Lo, Y.-F., Uchida, S., Huang, C.-L., Chen, B.-C.*; Cheng, C.-J.*, "Impairment in renal medulla development underlies salt wasting in Clc-k2 channel deficiency", JCI Insight , 9:151039 ; DOI:10.1172/jci.insight (2021) selected as the cover image
• Fan, Y.-J.*, Hsieh, H.-Y., Tsai, S.-F., Wu, C.-H., Lee, C.-M., Liu, Y.-T., Lu, C.-H., Chang, S.-W.*, Chen, B.-C.*, "Microfluidic channel integrated with a lattice lightsheet microscopic system for continuous cell imaging" Lab on a chip , 21, 344 DOI: 10.1039/d0lc01009j (2021)
• Tsai, Y.-C.; Tang, W.-C.; Low, C. S. L.; Liu, Y.-T.; Wu, J.-S.; Lee, P.-Y.; Lin, Y.-L.; Kanchanawong, P.; Gao, L, and Chen, B.-C.* "Rapid High Resolution 3D Imaging of Expanded Biological Specimens with Lattice Lightsheet Microscopy", Methods , 174, 11-19 DOI:10.1016/j.ymeth.2019.04.006 (2020)
• Chu, L.-A., Lu, C.-H., Yang, S.-M., Liu, Y-T, Feng, K.-L., Tsai, Y.-C., Chang, W.-K., Wang, W.-C., Chang, S.-W., Chen, P.; Lee, T.-K., Hwu, Y.-K., Chiang, A.-S.*; and Chen, B.-C.*, "Rapid single-wavelength lightsheet localization microscopy for clarified tissue" Nature Communications, 10, 4762, DOI: 10.1038/s41467-019-12715-3 (2019)
• Lu, C.-H.; Tang, W.-C.; Liu, Y.-T.; Wu, F. C. M.; Chen, C. Y.; Tsai, Y. C.; Yang, S.-M; Kuo, C.-W.; Okada, Y.; Hwu, Y.-K.; Chang, S.-W.; Chen, P. * and Chen, B.-C.*, "Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging." Communications Biology, 2, 177 DOI:10.1038/s42003-019-0403-9 (2019)
• Gao, L. *; Tang,W.-C.; Tsai, Y.-C.; Chen, B.-C.* "Lattice light sheet microscopy using tiling lattice light sheets" Opt. Express, 27 1497- 1506 DOI:10.1364/OE.27.001497 (2019)

• Tian, X.; Lin, T.-Y., L; Lin, P.-T., Tsai; M.-J., Chen, H.; Chen, W.-J.; Lee, C.-M.; Tu, C.-H.; Hsu, J.-C.; Hsieh, T.-H.; Tung, Y.-C.; Wang, C.-K.; Lin, S.; Chu, L.-A.; Tseng, F.-G.; Hsueh, Y.-P., Lee, C.-H.; Chen, P.; Chen, B.-C.* "Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassium acrylate-based expansion microscopy", Nature Communications, 15, 10911 DOI:10.1038/s41467-024-55305-8 (2024)
• Wang, U.-T. T.; Tian, X.; Liou, Y.-H.; Lee, S.-P.; Hu H.-T.; Lu, C.-H.; Lin, P.-T.; Cheng, Y.-J.; Chen P. and Chen, B.-C.* "Protein and lipid expansion microscopy with trypsin and tyramide signal amplification for 3D imaging", Scientific Reports, 13, 21922 DOI:10.1038/s41598-023-48959-9 (2023)
• Tang, W.-C.; Liu, Y.-T.; Yeh, C.-H.; Lin, Y.-L.; Lin, Y.-C.; Hsu, T.-L.; Gao, L.; Chen*, P. and B.-C. Chen*, "Optogenetic Manipulation of Cell Migration with High Spatiotemporal Resolution Using Lattice Lightsheet Microscopy" Communications Biology, 5, 879, DOI:10.1038/s42003-022-03835-6 (2022)
• Chu, L.-A., Lu, C.-H., Yang, S.-M., Liu, Y-T, Feng, K.-L., Tsai, Y.-C., Chang, W.-K., Wang, W.-C., Chang, S.-W., Chen, P.; Lee, T.-K., Hwu, Y.-K., Chiang, A.-S. *; and Chen, B.-C.*, "Rapid single-wavelength lightsheet localization microscopy for clarified tissue" Nature Communications, 10, 4762, DOI: 10.1038/s41467-019-12715-3 (2019)
• Lu, C.-H.; Tang, W.-C.; Liu, Y.-T.; Wu, F. C. M.; Chen, C. Y.; Tsai, Y. C.; Yang, S.-M; Kuo, C.-W.; Okada, Y.; Hwu, Y.-K.; Chang, S.-W.; Chen, P. * and Chen, B.-C.*, “Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging.” Communications Biology, 2, 177 DOI:10.1038/s42003-019-0403-9 (2019)