Overview
MINFLUX (MINimal photon FLUXes) is an innovative fluorescence microscopy technique developed by the team of Nobel Laureate Professor Stefan W. Hell. It combines the advantages of Stimulated Emission Depletion (STED) microscopy and Single-Molecule Localization Microscopy (SMLM) to achieve an unprecedented breakthrough in spatial localization precision in optical microscopy.
MINFLUX allows for nanometer-scale localization accuracy using only a minimal number of excitation photons, significantly reducing phototoxicity and photobleaching. Since localization does not require prolonged signal accumulation, it enables fast and dynamic single-molecule tracking.
Key Features:
- Spatial localization precision: < 3 nm in 2D, < 5 nm in 3D.
- Single-molecule dynamic tracking: < 100 µs per point, suitable for observing biomolecular dynamics.
- Extremely low phototoxicity and photobleaching, ideal for live-cell imaging.
- Supports 3D imaging and dual-color experiments (with two MINFLUX lasers: 560 nm & 640 nm).
- Self-operation
- Accompanied operation
- Pass the laboratory safety education for new colleagues of the Academia Sinica.
- Read the introduction, application process and management methods by yourself.
- After submitting the application form, the administrator will conduct machine training.
- Only after passing the assessment can you get access control and machine permissions; those who fail can apply for re-assessment one month later.
| National academic institutions | National non-academic institutions | International academic institutions | |
|---|---|---|---|
| Self-operation | NTD 1,000/h | - | USD 40/h |
| Accompanied operation | NTD 2,000/h | NTD 4,000/h | USD 80/h |
| Education and training | NTD 2,000/h | NTD 4,000/h | USD 80/h |
If you have research results produced using MINFLUX managed by this center, please add a note in the paper:
The MINFLUX imaging was supported by Research Center for Critical Issues (RCCI).
Contact: Mr. Di-yen Chueh
Mail
ken6552as.edu.tw
call
02-27873219
Manager: Dr. Shu-Yi Hsieh
Mail
shuyihsiehas.edu.tw
call
02-27873147